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T1ACR1_EYFP_pcDNA3.1

Plasmid

#161027

Purpose

Expresses strongly red-shifted T1ACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

T1ACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro

Plasmid

#177201

Purpose

encoding a dominant negative form of CEBP under inducible promoter

Depositor

Markus Covert

Article

Kudo et al bioRxiv (2022)

Insert

dnCEBP (A-C/EBP)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTE-0X38-GCT5

Plasmid

#27051

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-gfp

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only
pICH50581

Plasmid

#50256

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, act2 (AT3G18780, Arabidopsis thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pE-01

Plasmid

#166930

Purpose

Stop codon part (E)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

STOP CODON TAA and spacer - BLOCK E- Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pD-01

Plasmid

#166929

Purpose

TrrnB and L3S2P21 terminator part (D)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

DOUBLE TERMINATOR ( TrrnB - L3S2P21) _BLOCK D - Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pB-01

Plasmid

#166924

Purpose

Ptac promoter part (B)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

PTAC promoter - Block B- Flanked by BsaI restriction sites.

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pA-01

Plasmid

#166923

Purpose

LacI expression cassette (A)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

LacI expression cassette - Block A- Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFd031

Plasmid

#137970

Purpose

Expresses Prochlorococcus marinus MIT9211 Fd 1

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus MIT9211 ferredoxin 1

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMVS223

Plasmid

#176293

Purpose

SyntheticTranscriptionFactor_loxFAS_PGK_MCS_scarlett_ERD_ZIFDBD_P2A_Neo_loxP_AMP

Depositor

Max Staller

Article

Staller et al Cell Syst. 2022 Apr 20;13(4):334-345.e5.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTE-0X38-GCT1

Plasmid

#27053

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-gfp-ssrAec

Expression

Bacterial

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pARiBo4

Plasmid

#98893

Purpose

Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

Depositor

Pascale Legault

Article

Salvail-Lacoste et al RNA. 2013 Jul;19(7):1003-14. doi: 10.126

Insert

ARiBo4

Use

In vitro transcription vector

Promoter

T7 promoter

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLUM

Plasmid

#64169

Purpose

harbours marker genes LEU2, URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pDCB014

Plasmid

#135433

Purpose

Yeast integrating plasmid to express MCP-ABI and PYL1-VP64

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Inserts

MCP-ABI
PYL1-VP64

Expression

Yeast

Promoter

pAdh1 and pHHF2

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSB4A5 JQ929582

Plasmid

#38169

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

mKate2
Superfolder GFP

Use

Synthetic Biology; Biobrick cloning vector

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pUAST tdTomato mouse Rab35 WT

Plasmid

#53514

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Tags

tdtomato

Expression

Insect

Promoter

GAL4

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
BII-ChPtW

Plasmid

#133379

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-CBtW

Plasmid

#133372

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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TC690

Plasmid

#122619

Purpose

Punc4::QF2::QS::mKate

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

QF2::QS::mKate

Expression

Worm

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDUET-ctCPR-trAMO

Plasmid

#20112

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and truncated amorphadiene oxidase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only