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We narrowed to 12,188 results for: LEA

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Showing: 9481 - 9500 of 12188 results

  1. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid
    #202588
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Publication
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, changed O…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202588-plasmid-map-sequence-id-415632

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  6. pRSF-G1TMSNKRS

    Plasmid
    #198323
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Publication
    Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac
    Insert
    tRNA synthetase
    Expression
    Bacterial
    Promoter
    T7
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    198323-plasmid-map-sequence-id-393448

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  7. pET15b I-SceI (Ctermhis) K122I

    Plasmid
    #195614
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Publication
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Expression
    Bacterial
    Mutation
    C2458G, A2826T, A2827T
    Promoter
    T7
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195614-plasmid-map-sequence-id-383676

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  16. pmCell-Free_KA1_SARS-COV-2_S-frag3

    Plasmid
    #169403
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus N-term fragment of S protein after the furin cleavag with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    N-term frag. S protein after the furin cleavag (N SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169403-plasmid-map-sequence-id-336889

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  17. pmCellFree_KA1_SARS-COV-2_S-frag5

    Plasmid
    #169404
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus C-terminal ectodomain of S protein from the furin cleavag with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    C-term frag. S protein after the furin cleavag (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169404-plasmid-map-sequence-id-335424

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Showing: 9481 - 9500 of 12188 results