159,284 results

1. pTRK892

   Plasmid

   #71803

   Purpose

   Expression of mutated L. gasseri gusA from strong Ppgm promoter

   Depositor

   Rodolphe Barrangou, Todd Klaenhammer

   Article

   Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

   Insert

   gusA3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D573A

   Promoter

   Ppgm

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRUPATH Triple Gai3

   Plasmid

   #196050

   Purpose

   Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai3-RLuc8 (GNAI3 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

   Plasmid

   #83306

   Purpose

   Lentivirus vector to express guideRNA and dCas9 with puro resistant gene

   Depositor

   Tohru Kimura

   Article

   Murakami et al Nat Immunol. 2021 Mar;22(3):301-311. doi

   Insert

   sgRNA and dCas9 from pX330
   

   Use

   Lentiviral

   Tags

   FLAG and PA tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for sgRNA and CBh for dCas9

   Available since

   Dec. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. C1-F-tractin-mCherry

   Plasmid

   #155218

   Purpose

   Monitor global F-actin distribution

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2attPC

   Plasmid

   #51547

   Purpose

   DICE donor plasmid without neural transcription factors.

   Depositor

   Michele Calos

   Article

   Zhu et al Nucleic Acids Res. 2013 Dec 4.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP12102 - pAAV-AiE0387m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2102)

   Plasmid

   #208133

   Purpose

   Direct-expressing SYFP2 in astrocytes AAV Virus

   Depositor

   The Allen Institute for Brain Science, Boaz Levi

   Article

   Mich et al bioRxiv 2023.09.20.558718

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFC330

   Plasmid

   #87842

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   pyrG
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP14801 - pAAV-AiE0390m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4801)

   Plasmid

   #208163

   Purpose

   AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Insert

   iCreR297T
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRKH2

   Plasmid

   #71312

   Purpose

   Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

   Depositor

   Rodolphe Barrangou, Todd Klaenhammer

   Article

   O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

   Type

   Empty backbone

   Use

   E. coli-gram positive bacteria shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF Synaptophysin-mRuby

    Plasmid

    #188980

    Purpose

    Synaptic buton marker

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Synaptophysin (Syp Rat, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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