We narrowed to 16,183 results for: nes
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Plasmid#192142PurposeExpresses highly specific Sha2Cas9, and cloning backbone for sgRNADepositorInsertSha2Cas9-HF
UseAAVExpressionMammalianMutationSha2Cas9-HF (R247A and N415A)Available sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
141_pT3TS_iCre
Plasmid#171793PurposePlasmid for optimised iCre RNA synthesis using T3 promoter.DepositorInsertoptimised Cre for RNA expression
ExpressionBacterialPromoterT3Available sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-Cas9)
Plasmid#194768PurposeTo insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.DepositorInsertT2A-Cas9
UseCRISPRExpressionInsectAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJEP201-Lenti-TRE3G-Flag-GluN2B-WPRE-pA
Plasmid#164775PurposepLenti vector backbone designed to express Flag-GluN2B from a TRE3G promoter.DepositorInsertGluN2B
UseLentiviralTagsFlagAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP200-Lenti-TRE3G-Flag-GluN2A-WPRE-pA
Plasmid#164724PurposepLenti vector backbone designed to express Flag-GluN2A from a TRE3G promoter.DepositorInsertGluN2A
UseLentiviralTagsFlagAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSSa13
Plasmid#177371PurposeExpresses red-shifted luciferase under ScPGK1 promoter in Candida glabrataDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCgLUCopt-mut2
PromoterScPGK1pAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDSAY-vasaCas9sv40
Plasmid#173669PurposeCas9 expression vector for AnophelesDepositorInsertCas9 under control of vasa promoter and SV40 terminator
UseCRISPRExpressionInsectPromotervasaAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.mTagBFP2.let-7c
Plasmid#169310PurposeConstitutive overexpression of let-7c with mTagBFP2 as a reporter fluorescent proteinDepositorAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a Gx-NL2
Plasmid#169107PurposeExpresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc
Tagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pE-01
Plasmid#166930PurposeStop codon part (E)DepositorInsertSTOP CODON TAA and spacer - BLOCK E- Flanked by BsaI sites
UseSynthetic BiologyExpressionBacterialAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pD-01
Plasmid#166929PurposeTrrnB and L3S2P21 terminator part (D)DepositorInsertDOUBLE TERMINATOR ( TrrnB - L3S2P21) _BLOCK D - Flanked by BsaI sites
UseSynthetic BiologyExpressionBacterialAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pB-01
Plasmid#166924PurposePtac promoter part (B)DepositorInsertPTAC promoter - Block B- Flanked by BsaI restriction sites.
UseSynthetic BiologyExpressionBacterialAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET19b-DAD2 cPGFP(G164)
Plasmid#165993PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(G164)
ExpressionBacterialPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoBp-GFPuv
Plasmid#139073PurposeLow phosphate inducible promoter phoBp for GFP expressionDepositorInsertphoBp-GFPuv
ExpressionBacterialAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-ugpBp-GFPuv
Plasmid#139078PurposeLow phosphate inducible promoter ugpBp for GFP expressionDepositorInsertugpBp-GFPuv
ExpressionBacterialAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOGG113
Plasmid#133124PurposepL0M-PU-NoP, promotorless region used as negative controlDepositorInsertNoP promotorless region from plasmid pIJJ11268
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
kank4_pJet1.2
Plasmid#121982Purposefor generating kank4 riboprobes for WISH in zebrafishDepositorAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-14
Plasmid#119993PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mNeptune2.5DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmNeptune2.5
UsePcr templateTagsmNeptune2.5PromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-01
Plasmid#119980PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with moxBFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmoxBFP
UsePcr templateTagsmoxBFPPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-MDC
Plasmid#108950Purposeheterologous expression of MDC of S. cerevisiae in E. coliDepositorInsertMevalonate decarboxylase (MDC)
TagsHis6-TagExpressionBacterialAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only