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Anti-NSD1 [N312/10]

Plasmid

#190311

Purpose

Mammalian Expression Plasmid of anti-NSD1 (Human). Derived from hybridoma N312/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NSD1 (Homo sapiens) recombinant Mouse monoclonal antibody (NSD1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-NSD1 [N312/10-2b] - Chimeric

Plasmid

#227028

Purpose

Mammalian expression plasmid of anti-NSD1 (Human). Derived from hybridoma N312/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-NSD1 (Homo sapiens) recombinant mouse monoclonal antibody. (NSD1 Mouse)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 hygro

Plasmid

#98291

Purpose

Variant of lentiCRISPRv2 that confers hygromycin resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 neo

Plasmid

#98292

Purpose

Variant of lentiCRISPRv2 that confers G418 resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET-15b_LC3B

Plasmid

#73949

Purpose

For recombinant expression of human LC3B/MAP1LC3B in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

LC3B (MAP1LC3B Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pUC19_msfGFP-TCOF1-repair-template

Plasmid

#237632

Purpose

For knock-in msfGFP to TCOF1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TCOF1 (TCOF1 Human)

Use

CRISPR

Tags

msfGFP

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-pHluorin_M153R-CD63

Plasmid

#172117

Purpose

Fluorescent reporter for CD63-positive exosome secretion

Depositor

Alissa Weaver

Article

Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

Insert

CD63 (CD63 Human)

Use

Lentiviral

Tags

pHluorin_M153R in a small extracelluar loop of CD…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 blast

Plasmid

#98293

Purpose

Variant of lentiCRISPRv2 that confers blasticidin S resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-pHluorin_M153R-CD63-mScarlet

Plasmid

#172118

Purpose

Dual-color fluorescent reporter for CD63-positive exosome secretion and uptake

Depositor

Alissa Weaver

Article

Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

Insert

CD63 (CD63 Human)

Use

Lentiviral

Tags

mScarlet and pHluorin_M153R in a small extracellu…

Expression

Mammalian

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-myr-HA-Akt1

Plasmid

#46969

Purpose

lentiviral expression of myr-HA-Akt1

Depositor

Jialiang Wang

Article

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Tags

HA and myr

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CBA-hGBA1-cm-hGBA1-hGHpA

Plasmid

#218795

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

CMV-CBA-hGBA1-cm-hGBA1-hGHpA

Use

AAV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-hKDM4A-polyA

Plasmid

#101051

Purpose

in vitro transcription (T7 promoter)

Depositor

Yi Zhang

Article

Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

Insert

KDM4A (KDM4A Human)

Tags

Flag

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-hKDM4A(H188A)-polyA

Plasmid

#101052

Purpose

in vitro transcription (T7 promoter)

Depositor

Yi Zhang

Article

Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

Insert

KDM4A (KDM4A Human)

Tags

Flag

Expression

Mammalian

Promoter

T7

Available Since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-B

Plasmid

#104448

Purpose

Expression of human VAP-B fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Tags

EGFP and HA

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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p5E-gfap

Plasmid

#75024

Purpose

Can be used to drive expression in zebrafish astrocytes.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

glial fibrillary acidic protein promoter (gfap Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

gfap

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-A

Plasmid

#104447

Purpose

Expression of human VAP-A fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 puro

Plasmid

#98290

Purpose

Variant of lentiCRISPRv2 that confers puromycin resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-Sec61b-C1

Plasmid

#90992

Purpose

Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mEmerald-Sec61b (SEC61B Human, Synthetic)

Tags

mEmerald (green fluorescence)

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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IronFist

Plasmid

#243006

Purpose

Genetically encoded fluorescent iron reporter

Depositor

Roland Malli

Article

Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

Inserts

Hemerythrin-like (FBXL5 Human)
T2A
mCherry

Use

Gateway

Tags

mNeonGreen

Expression

Mammalian

Promoter

human phosphoglycerate kinase (hPGK)

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-Sec61b-C1

Plasmid

#90994

Purpose

Expresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mCherry-Sec61b (SEC61B Human, Synthetic)

Tags

mCherry (red fluorescence)

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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