Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 4,425 results for: Pet2;

Showing: 1411 - 1440 of 4425 results

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6xHis-CPL2/pET28a

Plasmid

#172095

Purpose

cDNA of CER11/CPL2 cloned into pET28a for protein expression

Depositor

George Haughn

Article

Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

Insert

CPL2 (CPL2 Mustard Weed)

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET28_6xHis-PARP1delZn1-L713F

Plasmid

#173937

Purpose

Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn1-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 1-96; L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn3-L713F

Plasmid

#173934

Purpose

Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-G871L

Plasmid

#174798

Purpose

Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-G871L (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

G871L, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A870L

Plasmid

#174797

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A870L (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A870L, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-K893I

Plasmid

#174799

Purpose

Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-K893I (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

K893I, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-43

Plasmid

#172759

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-43

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-35

Plasmid

#172755

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-35

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-19

Plasmid

#172748

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-19

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-3

Plasmid

#172740

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-3

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-12

Plasmid

#172744

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-12

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-14

Plasmid

#172745

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-14

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a Ec-iPGM-bio

Plasmid

#162575

Purpose

Expresses E. coli iPGM containing a C-terminal biotinylation site and His tag

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

E. coli iPGM bio-6His

Tags

6His tag, T7 tag, biotinylation sequence, and thr…

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_hTLN2-head-LBD

Plasmid

#164837

Purpose

Expression of human talin2 head mutant

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His8

Expression

Bacterial

Mutation

Lipid binding deficient mutations

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-GFP-tglG

Plasmid

#134679

Purpose

Expresses His6-GFP-TglG fusion in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglG

Tags

His6-GFP

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SznF E281A

Plasmid

#139591

Purpose

expresses SznF E281A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF E281A

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 281 to alanine

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SznF H225A

Plasmid

#139590

Purpose

expresses SznF H225A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF H225A

Tags

hexahistidine

Expression

Bacterial

Mutation

changed histidine 225 to alanine

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF E215A

Plasmid

#139565

Purpose

expresses SznF E215A in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF E215A

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 215 to alanine

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-DDX43-NT-G46D

Plasmid

#134588

Purpose

Express human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)

Tags

His

Expression

Bacterial

Mutation

full-length (G154D)

Promoter

T7

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-DDX43-FL-G84D

Plasmid

#134577

Purpose

Express human DDX43 mutant (full-length with G84D mutation) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-G84D (DDX43 Human)

Tags

His

Expression

Bacterial

Mutation

full-length (G84D)

Promoter

T7

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-Wing

Plasmid

#124552

Purpose

Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

The 5 residues making up the "wing" in …

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-Loop

Plasmid

#124551

Purpose

Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 216 to 223 replaced with corresponding s…

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes7/Imdes7

Plasmid

#107115

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des7

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes7/Imdes7

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes8/Imdes8

Plasmid

#107116

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des8

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes8/Imdes8

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes9/Imdes9

Plasmid

#107117

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des9

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes9/Imdes9

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes10/Imdes10

Plasmid

#107118

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des10

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes10/Imdes10

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes11/Imdes11

Plasmid

#107119

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des11

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes11/Imdes11

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes12/Imdes12

Plasmid

#107120

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des12

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes12/Imdes12

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes13/Imdes13

Plasmid

#107121

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des13

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes13/Imdes13

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes14/Imdes14

Plasmid

#107122

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des14

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes14/Imdes14

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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