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Plasmid#171081PurposeCo-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYCDepositorUseTagsGST and HisExpressionBacterialMutationPromoterT5 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only
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pET-28a (+)-hTFIIB
Plasmid#171084PurposeExpresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIBDepositorInsertTF2B (GTF2B Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-KIAA0947
Plasmid#49248PurposeMammalian expression of flag-tagged human KIAA0947DepositorInsertKIAA0947 (ICE1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-Med26
Plasmid#49250PurposeMammalian expression of flag-tagged Med26DepositorInsertMed 26 (MED26 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-hTBP
Plasmid#171073PurposeExpresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBPDepositorInsertTATA-box-binding protein (TBP Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-TAZ S89A
Plasmid#179911PurposeEntry vector of TAZ S89A for gateway coloning.DepositorInsertWW Domain Containing Transcription Regulator 1 (WWTR1 Human)
UseEntry vectorTags3X Flag-tagExpressionMutationSerine 89 mutated to AlaninePromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCHC030 (∆CBBp ∆MBH ∆SH)
Plasmid#226205PurposeElectroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).DepositorInsertHomology arms for CBBp, MBH, and SH operons, and intervening sequencessequences.
UseSynthetic BiologyTagsExpressionMutation∆cbbR ∆cbbLSXYEFPTZGKA ∆hoxKGZMLOQRTV ∆hypA1B1F1C…PromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Syn-ChrimsonR-tdT
Plasmid#59171PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH-TAZ S89A
Plasmid#179910PurposeConstruct for expressing an activated form of TAZ (encoding be gene WWTR1)DepositorInsertWW Domain Containing Transcription Regulator 1 (WWTR1 Human)
UseTags3X Flag-tagExpressionMammalianMutationSerine 89 mutated to AlaninePromoterEF1aAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-MIOS(1-875) in pRK5
Plasmid#184561PurposeCMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells.DepositorInsertMIOS (MIOS Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn GFP-Fxr1
Plasmid#112732PurposeAAV plasmid that expresses GFP-Fxr1 fusion protein under hSYN promoterDepositorInsertFxr1p (Fxr1 Mouse)
UseAAVTagsGFPExpressionMammalianMutationPromoterhSYNAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLEX-fwd[Chrimson-tdT]
Plasmid#59137PurposeExpression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)DepositorInsertChrimson-tdTomato
UseCre/LoxTagstdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-F-ELL2
Plasmid#49422PurposeMammalian expression of flag-tagged human ELL2DepositorInsertELL2 (ELL2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-EAF1
Plasmid#49249PurposeMammalian expression of flag-tagged human EAF1DepositorInsertEAF1 (EAF1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-EAF2
Plasmid#49251PurposeMammalian expression of flag-tagged human EAF2DepositorInsertEAF2 (EAF2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-F-Med26-K74A K75A
Plasmid#49253PurposeMammalian expression of flag-tagged human Med26-K74A K75ADepositorInsertMed 26 (MED26 Human)
UseTagsHAExpressionMammalianMutationK74A K75APromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-F-Med26-NTD
Plasmid#49255PurposeMammalian expression of flag-tagged Med26-NTDDepositorInsertMed 26 (MED26 Human)
UseTagsFlagExpressionMammalianMutationNTD (aa 1-339)PromoterAvailable sinceDec. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-F-Med26-R61A K62A
Plasmid#49254PurposeMammalian expression of flag-tagged human Med26 R61A K62ADepositorInsertMed26 (MED26 Human)
UseTagsFlagExpressionMammalianMutationR61A K62APromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-iSAM
Plasmid#211495PurposeAAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRaDepositorInsertsUniSAM insert
TET-ON
Neo Resistance
Insulator
UseCRISPR and Synthetic BiologyTagsT2A-mCherry TagExpressionMammalianMutationPromoterEF1a, NA, Splicing acceptor, and TREGV promoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIF
Plasmid#171082PurposeCo-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permissionDepositorUseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only