Addgene

We narrowed to 2,075 results for: Pam

Showing: 941 - 960 of 2075 results

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pMXS-IRES-BLAST flag-CAD R2187A

Plasmid

#169827

Purpose

Expresses C-terminal flag-tagged CAD with mutation at reported trimerization interface of the ATCase domain of CAD

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

R2187A, T456A S1406A S1859A; TCCC -> AGTC sile…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD M1601E

Plasmid

#169828

Purpose

Expresses C-terminal flag-tagged CAD with mutation at reported dimerization interface of the DHOase domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

M1601E; TCCC -> AGTC silent mutations at nt527…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD H1734A H1741A

Plasmid

#169830

Purpose

Expresses C-terminal flag-tagged CAD with mutations at reported dimerization interface of the DHOase domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

H1734A H1741A; TCCC -> AGTC silent mutations a…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD delta Loop1

Plasmid

#169831

Purpose

Expresses C-terminal flag-tagged CAD with deletion of amino acids F1366 - P1376 in allosteric domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

Deletion of amino acids F1366 - P1376; TCCC ->…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD delta Loop2

Plasmid

#169832

Purpose

Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 in allosteric domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

Deletion of amino acids R1398 - S1407; TCCC ->…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD S1406A

Plasmid

#169820

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1406A; TCCC -> AGTC silent mutations at nt527…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD S1859E

Plasmid

#169822

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1859E; TCCC -> AGTC silent mutations at nt527…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD T456A S1406A

Plasmid

#169823

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1406A; TCCC -> AGTC silent mutations at…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD T456A S1859A

Plasmid

#169824

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1859A; TCCC -> AGTC silent mutations at…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCBE3-NG-HF1

Plasmid

#218160

Purpose

This plasmid harbors the base editor SCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

Depositor

Yuhui Sun

Article

Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

Inserts

scbe3-NG-HF1
a programmable sgRNA cloning cassette

Use

CRISPR; Sgrna transcription

Tags

Expression

Bacterial

Mutation

Mutations in the portion of SpCas9 : D10A / N497A…

Promoter

rpsL promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCBE3-NG-Hypa

Plasmid

#218161

Purpose

This plasmid harbors the base editor SCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

Depositor

Yuhui Sun

Article

Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

Inserts

scbe3-NG-Hypa
a programmable sgRNA cloning cassette

Use

CRISPR; Sgrna transcription

Tags

Expression

Bacterial

Mutation

Mutations in the portion of SpCas9 : D10A / N692A…

Promoter

rpsL promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCBE3-NG-HF1-Hypa

Plasmid

#218162

Purpose

This plasmid harbors the base editor SCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

Depositor

Yuhui Sun

Article

Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

Inserts

scbe3-NG-HF1-Hypa
a programmable sgRNA cloning cassette

Use

CRISPR; Sgrna transcription

Tags

Expression

Bacterial

Mutation

Mutations in the portion of SpCas9 : D10A / N497A…

Promoter

rpsL promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonor-Prf1-ires-mCherry

Plasmid

#209073

Purpose

Targeting vector for the mouse Prf1 locus to correct prf1 gene

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

Prf1-ires-mCherry HDR template (parts of Prf1 with mutated PAM) (Prf1 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonor-Prf1-mock-ires-mCherry

Plasmid

#209074

Purpose

Targeting vector for the mouse Prf1 locus to correct prf1 gene

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

Prf1-mock-ires-mCherry HDR template (parts of Prf1(S399Stop) with mutated PAM) (Prf1 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD L1405A

Plasmid

#188136

Purpose

Expresses C-terminal flag-tagged human CAD L1405A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD A1400E

Plasmid

#188137

Purpose

Expresses C-terminal flag-tagged human CAD A1400E in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD C1374S

Plasmid

#188139

Purpose

Expresses C-terminal flag-tagged human CAD C1374S in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD E1367A

Plasmid

#188140

Purpose

Expresses C-terminal flag-tagged human CAD E1367A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD E1373A

Plasmid

#188142

Purpose

Expresses C-terminal flag-tagged human CAD E1373A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD D1371A

Plasmid

#188145

Purpose

Expresses C-terminal flag-tagged human CAD D1371A in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCC -> AGT silent mutations at nt439-441 elimi…

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 941 - 960 of 2075 results

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