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pCW57.1-Neurod1-3xT7

Plasmid

#200890

Purpose

Inducible lentiviral expression of Neurod1

Depositor

Kai Ge

Article

Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

Insert

Neurod1 (Neurod1 Mouse)

Use

Lentiviral; Doxycycline inducible

Tags

3xT7

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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LAMP1-mCherry-FRB*

Plasmid

#207145

Purpose

Lysosome-targeted rapalog dimerizer

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

LAMP1 (LAMP1 Human)

Use

Tags

FRB* (T2098L mutant of FKBP-rapamycin binding dom…

Expression

Mammalian

Mutation

Silent mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCD4-TagRFP-T (RHPMP151)

Plasmid

#119238

Purpose

Expresses CD4 fused to TagRFP-T

Depositor

Ricardo Henriques

Article

Pereira et al Front Immunol. 2019 Apr 5;10:675. doi: 1

Insert

CD4 (CD4 Human)

Use

Tags

tagRFP-T

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ (S89A)

Plasmid

#24815

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Serine 89 mutated to Alanine

Promoter

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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Venus-G protein Beta2 in pcDNA3.1

Plasmid

#42182

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

G protein Beta 2 (GNB2 Human)

Use

Tags

Venus(156-239)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
3XFlag pCMV5-TOPO TAZ (∂WW)

Plasmid

#24811

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of amino acids 111-158.

Promoter

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
3XFlag pCMV5-TOPO TAZ ∂CC

Plasmid

#24816

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of amino acids 222-265 (coiled coil doma…

Promoter

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
3XFlag pCMV5-TOPO TAZ (∂304)

Plasmid

#24810

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Contains ONLY amino acids 1-304.

Promoter

Available since

June 3, 2010

Availability

Industry, Academic Institutions, and Nonprofits
3XFlag pCMV5-TOPO TAZ (106∂)

Plasmid

#24814

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of aa 1-106

Promoter

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pCI-neo-myc-LC3-HA

Plasmid

#45244

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Ago2_1

Plasmid

#73529

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Ago2_2

Plasmid

#73530

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
3XFlag pCMV5-TOPO TAZ (∂393)

Plasmid

#24812

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Contains ONLY amino acids 1-393. (No PDZ binding…

Promoter

Available since

June 3, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pCI-neo-myc-LC3(G120A)-HA

Plasmid

#45245

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Glycine at position 120 mutated to Alanine

Promoter

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3_shRNA resistant PKA-Cα-mEGFP

Plasmid

#114143

Purpose

Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pRS405 PEX14-VAC8-RFP

Plasmid

#166844

Purpose

Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, PEX14 Budding Yeast)

Use

Tags

PEX14 transmembrane domain and RFP

Expression

Yeast

Mutation

PEX14 transmembrane domain

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo-myc-LC3(deltaC22)

Plasmid

#45448

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

Encodes amino acids 1-120

Promoter

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3-PKA-RIIβ-sREACh

Plasmid

#114113

Purpose

Mammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

PKA regulatory subunit RII beta tagged by sREACh (low-irradiant YFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP2A-siRNAres

Plasmid

#115332

Purpose

expresses siRNA-resistant mCherry-CHMP2A in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2A (CHMP2A Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

mutated to be resistant to siRNA knockdown

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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FB007

Plasmid

#119705

Purpose

Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Promoter gpdA

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mutation

Promoter

gpdA

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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