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Plasmid#195051Purposeintegration cassette for ku70 deletion and expression of NAT gene under pGPD for characterizationDepositorInsertNAT
UseTagsExpressionBacterialMutationPromoterpGPDAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)
Plasmid#195050Purposeintegration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterizationDepositorInsertNAT
UseTagsExpressionBacterialMutationPromoterpADH2Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(F) pXYL (G) P1 (SBE216)
Plasmid#195017Purposepromoter of the gene xylose reductase from R. toruloides for the position P2 (F/G)DepositorInsert(F) pXYL (G)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(R) pXYL (D) P1 (SBE215)
Plasmid#195016Purposepromoter of the gene xylose reductase from R. toruloides for the position P1 (R/D)DepositorInsert(R) pXYL (D)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(R)pADH2(D)_P1(SBE214)
Plasmid#195015Purposepromoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1DepositorInsert(B) pADH2 (R)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) NAT marker (R) (SBE173)
Plasmid#195014Purposenourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+NAT+tNOS
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(I) pGPD (J)_P3 (SBE111)
Plasmid#194990Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P3 position (I/J)DepositorInsert(I) pGPD (J)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(S) insD_ku70 (Q) (SBE139)
Plasmid#187627Purposethe insertional region downstream for the deletion of the KU70 gene in R. toruloides. Overhangs S/QDepositorInsert(S) insD KU70 (Q)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(P) insUP_ku70 (SBE138) (B)
Plasmid#187626Purposethe insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/BDepositorInsert(P) insUP KU70 (B)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K3.loxP
Plasmid#63202PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5.loxP
Plasmid#63204PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO3.loxP
Plasmid#63205PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO6
Plasmid#63211PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAIP4_1UXZ
Plasmid#234166PurposeHeterologous protein expression of cellulase B in Escherichia coliDepositorInsert1UXZ
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHIE096 CMV Bxb1
Plasmid#139252PurposeEncodes a Bxb1 recombinase for Landing Pad integration.DepositorInsertBxb1 Recombinase
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1019 TRE3GV DsRedExpress2 in TU2
Plasmid#139254PurposeTRE3GV promoter and DsRedExpress2 in TUPV2DepositorInsertDsRedExpress2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM467 TRE3GV EYFP in TU3
Plasmid#139255PurposeTRE3GV promoter and EYFP in TUPV3DepositorInsertEYFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_3S60
Plasmid#234231PurposeHeterologous protein expression of lectin OAA in Escherichia coliDepositorInsert3S60
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 7, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_5M2Y
Plasmid#234193PurposeHeterologous protein expression of B7LG64_ECO55 in Escherichia coliDepositorInsert5M2Y
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_3H6Q
Plasmid#234185PurposeHeterologous protein expression of macrocypin-1a in Escherichia coliDepositorInsert3H6Q
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits