We narrowed to 1,241 results for: post

1. pCS3-MT-BX/ARF65-132

   Plasmid

   #78763

   Purpose

   To overexpress ARF65-132 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

   Insert

   ARF (CDKN2A Human)
   

   Use

   Tags

   6xMYC

   Expression

   Mammalian

   Mutation

   65-132

   Promoter

   CMV IE94

   Available Since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pEGFP-PTEN-deltaPDZ

   Plasmid

   #110182

   Purpose

   Expression of a mutated PTEN lacking its 4 last aa, and tagged with EGFP

   Depositor

   Jose Esteban

   Article

   Jurado et al EMBO J. 2010 Aug 18;29(16):2827-40. doi:

   Insert

   PTEN (Pten Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of last 4 amino acids

   Promoter

   CMV

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pgex6p-1mciap1mut

   Plasmid

   #11484

   Depositor

   Jon Ashwell

   Article

   Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

   Insert

   mciap1mut (Birc2 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   582 to alanine

   Promoter

   Available Since

   March 8, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pT2/shCol1a1/Scarlet_Seq1.2

   Plasmid

   #201404

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2/shCol1a1/Scarlet-Seq1.1

   Plasmid

   #201401

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2/Col1a1/GFP4_Seq 1.1

   Plasmid

   #201400

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-hFAM3B-HA

   Plasmid

   #180145

   Purpose

   Coding sequence of human FAM3B pCS2 with C terminal HA tag

   Depositor

   Edward De Robertis

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

   Insert

   FAM3B (FAM3B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2-hFAM3B-FLAG

   Plasmid

   #180146

   Purpose

   Coding sequence of human FAM3B in pCS2 with C terminal FLAG tag

   Depositor

   Edward De Robertis

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

   Insert

   FAM3B (FAM3B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSicoR (EGFP) shPnky-2

   Plasmid

   #79142

   Purpose

   Stable expression of shRNA targeting mouse Pnky. The shRNA (and EGFP) can be excised by the addition of Cre

   Depositor

   Daniel Lim

   Article

   Ramos et al Cell Stem Cell. 2015 Apr 2;16(4):439-47.

   Insert

   shPnky-2 (Gm30731 Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for the shRNA and CMV for EGFP

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-HDR-mEGFP-camk2a

    Plasmid

    #104589

    Purpose

    AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse camk2a

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

    Plasmid

    #118273

    Purpose

    pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-GFP-IRES-hSNCA (A30P)

    Plasmid

    #185720

    Purpose

    AAV expression of GFP and human α-Synuclein with A30P mutation from hSyn promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    synuclein, alpha (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Changed Ala 30 to Pro

    Promoter

    hSyn

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIA123

    Plasmid

    #234038

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.

    Depositor

    Craig Ellermeier

    Article

    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pgdh and Pxyl

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4/TO/GFPZNF598-9P-9A

    Plasmid

    #141192

    Purpose

    Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    ZNF598 (ZNF598 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    all 3 proline repeats are mutated to Alanine (9xP…

    Promoter

    CMV

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-HDR-mEGFP-Actin

    Plasmid

    #119870

    Purpose

    AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta Actin

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    beta Actin (Actb Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4/TO/GFP-TTP

    Plasmid

    #141190

    Purpose

    Expresses GFP-TTP in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    TTP (ZFP36 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMTS_mScarlet-i_N1

    Plasmid

    #85059

    Purpose

    In vivo visualization of the mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    MTS (COX8A Human)
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMTS_mScarlet_N1

    Plasmid

    #85057

    Purpose

    In vivo visualization of the mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    MTS (COX8A Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. FHL-cpmTq2-Calcium-lifetime-sensor

    Plasmid

    #129628

    Purpose

    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    FHL-cpmTq2-Calcium-lifetime-sensor
    

    Use

    Tags

    6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV,&Rhamnose

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. retro-puro vector

    Plasmid

    #58250

    Purpose

    Retroviral expression vector encoding an empty IRES-Puromycin cassette

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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