We narrowed to 34,123 results for: CMV

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pCMV-Parkin(C431N)-A92mKO2

Plasmid

#213545

Purpose

Transient mammalian expresssion of ligase-dead C431N Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Publication

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Expression

Mammalian

Mutation

C431N mutation in Parkin

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-TALE-R-JAK2-Ddd9-C

Plasmid

#204854

Purpose

For DdCBE editing using Ddd9 at JAK2 site in human cells

Depositor

Caixia Gao

Publication

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

Ddd9-C

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TALE-L-JAK2-Ddd9-N

Plasmid

#204853

Purpose

For DdCBE editing using Ddd9 at JAK2 site in human cells

Depositor

Caixia Gao

Publication

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

Ddd9-N

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-ZNF354C_mutant_M41K-L42K-E43K

Plasmid

#194185

Purpose

Expresses ZNF354C with mutated KRAB domain (M41K, L42K, E43K mutation)

Depositor

Ralf Brandes, Matthias Leisegang

Publication

Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

Insert

ZNF354C (ZNF354C Human)

Tags

Myc-DDK

Expression

Mammalian

Mutation

KRAB domain mutant M41K/L42K/E43K

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP2-IRES-HO1-N1

Plasmid

#178967

Purpose

Expresses miRFP2 and HO1 in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2

Tags

HO1 and no

Expression

Mammalian

Mutation

NO

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP670-eDHFR(69K6)

Plasmid

#178859

Purpose

Mammalian expression of miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Publication

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S22A S23A-msfGFP

Plasmid

#180338

Purpose

mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Publication

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S22A S23A

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S12A S13A-msfGFP

Plasmid

#180337

Purpose

mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Publication

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S12A S13A

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Armc12/FLAG/BioID2/HA

Plasmid

#180498

Purpose

Expression vector of BirA-fused mouse Armc12. HA-tagged BirA was linked to the FLAG-tagged Armc12. See Fig.S6 of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

Depositor

Masahito Ikawa

Publication

Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

Insert

armadillo repeat containing 12 (Armc12 Mouse)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP670-Zdk1-MCS

Plasmid

#178873

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of miRFP670-Zdk1

Depositor

Shinya Tsukiji

Publication

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

miRFP670-Zdk1

Expression

Mammalian

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-Zdk1-Vav2cat

Plasmid

#178875

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to mCherry-Zdk1

Depositor

Shinya Tsukiji

Publication

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

mCherry-Zdk1-Vav2(DH-PH-CRD) (Vav2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 allKR

Plasmid

#129298

Purpose

Expresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells

Depositor

Robin Ketteler

Publication

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed all 22 Lysine residues to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 K295R

Plasmid

#129302

Purpose

Expresses pCMV 3xFLAG-ATG3 K295R in mammalian cells

Depositor

Robin Ketteler

Publication

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Lysine 295 to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (G20V)

Plasmid

#119308

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cells

Depositor

Peter Howley

Publication

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (G20V) (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

G20V

Promoter

CMV

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (C21Y)

Plasmid

#119304

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cells

Depositor

Peter Howley

Publication

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (C21Y) (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

C21Y

Promoter

CMV

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 55-376)

Plasmid

#119313

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 55-376 in mammalian cells

Depositor

Peter Howley

Publication

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 55-376

Promoter

CMV

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (R39H)

Plasmid

#119309

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I (R39H) in mammalian cells

Depositor

Peter Howley

Publication

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (R39H) (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

G20V

Promoter

CMV

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 55-852)

Plasmid

#119314

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 55-852 in mammalian cells

Depositor

Peter Howley

Publication

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 55-852

Promoter

CMV

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4A RNAiR

Plasmid

#115172

Purpose

expresses RNAi-resistant Sema4A with N-terminal myc tag

Depositor

Suzanne Paradis

Publication

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

Sema4A (Sema4a Mouse)

Tags

myc

Expression

Mammalian

Mutation

Silent mutation between amino acids in ORF (401-4…

Promoter

CMV

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/SP S245X with 5'UTR

Plasmid

#92370

Purpose

expression of 245X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Publication

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

S245X

Promoter

CMV

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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