We narrowed to 79,196 results for: rest
-
Plasmid#125790Purposeconstitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)DepositorAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
pLX209-neo-WRN E84A
Plasmid#125789Purposeconstitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)DepositorAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-FLEX-SaCas9-U6-sgGrin1
Plasmid#124852PurposeMutagenesis of Grin1DepositorInsertGrin1 (Grin1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK364 (CMV-OsTIR1-loxP-PURO-loxP)
Plasmid#121184PurposeOsTIR1 introductionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCMV-OsTIR1-loxP-PURO-loxP
UseCRISPRMutationOsTIR1 codon optimized for human cellsAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFPC1-hVAP-B KD/MD
Plasmid#104450PurposeExpression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertvesicle-associated membrane protein-associated protein B (VAPB Human)
TagsEGFP and HAExpressionMammalianMutationchanged K87 to D and M89 to DAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final
Plasmid#107009PurposeModified Luciferase expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD274 (CD274 Human)
UseLuciferaseExpressionMammalianAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hNedd4-2
Plasmid#83433Purposefull length Nedd4L with His Xpress tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman Nedd4L with C2 domain (NEDD4L Human)
TagsHis Tag (6x) and Xpress Epitope TagExpressionMammalianPromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC-] (AVA2515)
Plasmid#85443PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1S-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73032PurposeExpress fluorescent and luminescence dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseExpressionMammalianPromoterTruncated EF1Available sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR
Plasmid#60227PurposeExpresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.DepositorInsertssgRNA
Cre recombinase
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HAExpressionMammalianPromoterCBh and U6Available sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CRY2(D387A)-mCherry
Plasmid#58369PurposeLight insensitive CRY2 mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRY2[1-498] (D387A) (CRY2 Mustard Weed)
TagsmCherryExpressionMammalianMutationD387APromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSP64-mHCN2
Plasmid#53060PurposeExpresses alpha subunit of mouse HCN2 channel in Xenopus oocytesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPotassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)
UseXenopus oocyte expressionMutationE55G, R237H, and K283R polymorphisms compared to …PromoterSP6Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX Grb2-SH2
Plasmid#46440DepositorInsertGrb2 (GRB2 Human)
TagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 58-159PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
XE248 pCDNA3.1+zeo-VSV-KLHL12
Plasmid#16761DepositorAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pClneoMyc human NDR1
Plasmid#37023DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNDR1 (STK38 Human)
TagsmycExpressionMammalianPromoterCMVAvailable sinceMay 23, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMCS.71/2272.Hygro
Plasmid#22731DepositorInsertLox71-FRT-PGK-hygro-FRT-Lox2272
UseCre/LoxMutationThis is a basal exchange vector for RMCE. The vec…Available sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134A
Plasmid#69276PurposeGolden Gate entry vector; 4th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
T4 td upstream intron-PABP
Plasmid#189972PurposePart 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAsDepositorInsertT4 td upstream intron-PABP
UseCloningPromoterN/AAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS428
Plasmid#11257DepositorTypeEmpty backboneExpressionYeastAvailable sinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only