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pKL11-GCaMP6f bb100

Plasmid

#158983

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

Depositor

Joel Kralj

Publication

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23100 (sigma 70)

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HsSPHK1

Plasmid

#118091

Purpose

Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

Depositor

Kevin Lynch

Publication

Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

Insert

Homo sapiens Sphingosine Kinase 1

Tags

FLAG

Expression

Yeast

Promoter

GAL1, 10

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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MmSphk1

Plasmid

#118599

Purpose

Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice

Depositor

Kevin Lynch

Publication

Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

Insert

Mus musculus sphingosine kinase 1

Tags

FLAG

Expression

Yeast

Promoter

GAL1, 10

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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GST-RNF126

Plasmid

#138643

Purpose

Bacterial expression of GST-RNF126 fusion protein

Depositor

Jane McGlade

Publication

Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

Insert

RNF126 (RNF126 Mouse)

Tags

GST

Expression

Bacterial

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRAB18-GFP

Plasmid

#106984

Purpose

ABA signaling reporter, GFP under the RAB18 promoter

Depositor

Julian Schroeder

Publication

Kim et al Curr Biol. 2011 Jun 7;21(11):990-7. doi:

Insert

sGFP

Expression

Plant

Promoter

RAB18

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-hphMX6

Plasmid

#105159

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-hphMX6

Tags

GBP

Expression

Bacterial and Yeast

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-mEGFP

Plasmid

#103979

Purpose

Plasmid encoding mEGFP

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEGFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-HT/spoR

Plasmid

#73631

Purpose

Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Publication

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

guanylate kinase

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_tdTomato

Plasmid

#62407

Purpose

MXS_chaining vector with tdTomato

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tdTomato

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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p6239 pGL4-HPV16-LCR (nt 7000-85)-luciferase

Plasmid

#32889

Depositor

Peter Howley

Publication

Ottinger et al Virology. 2009 Dec 20;395(2):161-71.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LCR HPV16

Promoter

LCR

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pShGSK3bE

Plasmid

#37526

Depositor

Jerry Crabtree

Publication

Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GSK3b (GSK3B Human)

Tags

HA

Expression

Mammalian

Promoter

SRa promoter

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn S87E

Plasmid

#36059

Depositor

Hilal Lashuel

Publication

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87E (SNCA Human)

Expression

Bacterial

Mutation

S87E

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pMN cFKT

Plasmid

#18785

Depositor

Han Htun

Publication

Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

Insert

TRAP (mtrB Bacillus subtilis)

Tags

6xHis and HA

Expression

Mammalian

Available since

Aug. 12, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-CMV-PdCO-mScarlet

Plasmid

#198507

Purpose

Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

Depositor

Ofer Yizhar

Publication

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PdCO

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

CMV

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pmStayGold-Fb(ALFA)

Plasmid

#249309

Purpose

pmStayGold-Fb(ALFA) expression in mammalian cells.

Depositor

Vladislav Verkhusha

Publication

Barykina et al Nat Methods. 2026 Apr 22. doi: 10.1038/s

Insert

pmStayGold-Fb(ALFA)

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-UbC-CytoTape-vivo-HA

Plasmid

#250670

Purpose

CytoTape-vivo structural monomer for CytoTape-vivo fiber formation and elongation for mouse brain in vivo applications

Depositor

Changyang Linghu

Publication

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-vivo-HA

Use

AAV

Expression

Mammalian

Promoter

UbC promoter

Available since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-c-fos-CytoTape-V5

Plasmid

#239429

Purpose

CytoTape signal monomer for recording c-fos promoter transcriptional activity

Depositor

Changyang Linghu

Publication

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-V5

Use

AAV

Tags

V5-dMBP

Expression

Mammalian

Promoter

c-fos promoter

Available since

Oct. 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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EcoHIV-mScarlet

Plasmid

#235598

Purpose

EcoHIV-mScarlet is a modification of pNL4-3-EcoHIV (Potash et al, PNAS 2005). EcoHIV is rodent-infectable modification of HIV where HIV env replaced for MLV-env

Depositor

Michael Shtutman

Publication

Li et al J Vis Exp. 2024 Dec 20;(214). doi: 10.37

Insert

NL4-3 EcoHIV mScarlet

Use

Lentiviral

Tags

mScarlet

Available since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-hTFIIB

Plasmid

#171084

Purpose

Expresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIB

Depositor

Joan Conaway

Publication

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

TF2B (GTF2B Human)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-IRF3

Plasmid

#221260

Purpose

Expression of IRF3 in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRF3 (Irf3 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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