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pBbB5a-GFP

Plasmid

#35352

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pJUMP46-2A(sfGFP)

Plasmid

#126990

Purpose

Level 2 vector. sfGFP reporter. Origin p15A (medium copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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yfpsyndat

Plasmid

#19991

Depositor

Jonathan Javitch

Article

Carvelli et al J Neurochem. 2002 May . 81(4):859-69.

Insert

yfpsynhdat (SLC6A3 Human)

Tags

yfp

Expression

Mammalian

Available Since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pGL3Basic-Mdm2-T3

Plasmid

#32369

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pFRETtv-2in1-NC

Plasmid

#105122

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mTRQ2 and mVenus

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pYM28-BirA*

Plasmid

#160290

Purpose

Used for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: HIS3

Depositor

Martin Ott

Article

Salvatori et al Mol Cell. 2020 Feb 20;77(4):887-900.e5.

Insert

BirA*

Expression

Yeast

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt2b

Plasmid

#42275

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt2b (Wnt2b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
EphA1-RTK-Cry2olig

Plasmid

#176601

Purpose

EphrinA1 RTK fusion to Cry2olig - RTK domain only

Depositor

Robert Hughes

Article

Wurz et al bioRxiv [Preprint]. 2024 Feb 7:2024.02.0

Insert

EphA1rtk-Cry2olig-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMEH275 NLS dTomato

Plasmid

#168210

Purpose

Mammalian expression of nuclear-localized dTomato

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

NLS dTomato

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 - mSRSF6

Plasmid

#120420

Purpose

overexpression of mouse Srsf6

Depositor

Gillian Bates, Andreas Neueder

Article

Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

Insert

SRSF6 (Srsf6 Mouse)

Expression

Mammalian

Promoter

CMV immediate/early

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pPSmOrange-Tubulin

Plasmid

#31919

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Tags

Tubulin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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10xUAS-GFP-DD

Plasmid

#104989

Purpose

Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

Depositor

Jing-W Wang

Article

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized GFP (GFP-DD)

Tags

DD

Expression

Insect

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODAL

Plasmid

#115638

Purpose

For targeted integration and inducible expression of human NODAL using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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N-term Hec1 Plk1 FRET sensor c-jun substrate

Plasmid

#45204

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Hec1-targeted Plk1 FRET sensor

Tags

CyPET, YPET

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pYPQ151

Plasmid

#69302

Purpose

Gateway entry vector with pcoCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9D10A (Plant codon-optimized)

Use

CRISPR

Tags

2XFLAG

Mutation

D10A mutation; NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBbS1c-RFP

Plasmid

#35338

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Trc

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-TALE1

Plasmid

#27969

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE1

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)GFP-SynaptophysW

Plasmid

#27235

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

Synaptophys-GFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pGL3Basic-Mdm2-T4

Plasmid

#32370

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
MCV

Plasmid

#81174

Purpose

MCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 cloning of inserts

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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