We narrowed to 18,860 results for: REV

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Stx3-pmCitrine

Plasmid

#92421

Purpose

Expression of syntaxin-3 C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx3 (Stx3 Rat)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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hSyn-GW-dCas9-SET2-IRES2-mCherry

Plasmid

#202089

Purpose

Neuron-specific expression vector of dCas9-Set2

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2

Tags

3x FLAG and mCherry

Expression

Mammalian

Promoter

human Synapsin

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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UKNP1.2.1

Plasmid

#97356

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3GLB1-1/1

Plasmid

#91346

Purpose

Protein expression and purification of human SH3 domain construct SH3GLB1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3GLB1-1/1 (SH3GLB1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-At_PRORP2-His

Plasmid

#67870

Purpose

bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP2 (PRORP2 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCT

Plasmid

#60620

Purpose

Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

Depositor

Huimin Zhao

Article

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pXNgate21-3HA

Plasmid

#105078

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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prp8-G736E

Plasmid

#108450

Purpose

prp8-G736E in pRS313 backbone for expression in yeast

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2019 Feb 5;116

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

changed G736 to E

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM3-C71,72,105A

Plasmid

#58396

Purpose

Expressed murine IFITM3-C71,72,105A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteines 71, 72, and 105 to Alanine

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pAAV Fos YFP MS2

Plasmid

#131775

Purpose

Fos upstream msfYFP 24xMS2 Fos downstream for CRISPR tagging

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

msfYFP - 24x MS2 loops

Use

AAV and CRISPR

Promoter

n/a

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pECFP-Rac1Y40C

Plasmid

#105293

Purpose

Control for FRET biosensor analysis - cannot bind downstream effectors

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Rac1 (RAC1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Y40C

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-223

Plasmid

#26334

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-223 (Mir223 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
PE_CO-Mini-N

Plasmid

#190336

Purpose

Expressing the N-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, ngRNA and pegRNA for PCSK9 +1A insertion

Depositor

Rasmus Bak

Article

Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

Insert

PE_CO-Mini-N, ngRNA and pegRNA for PCSK9 +1A insertion

Use

AAV

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKH143_EGFP-YIPF3 LIR mut

Plasmid

#214975

Purpose

GFP tagged LIR mutant YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GFP-YIPF3_mut (YIPF3 Human)

Use

Lentiviral

Mutation

LIR mutant

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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mGFAP-Halo

Plasmid

#169474

Purpose

the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

GFAP (Gfap Mouse)

Tags

HaloTag

Expression

Mammalian

Mutation

Changed R 236 to H

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRPL28(K55M) SpHIS5 (pNTI666)

Plasmid

#115436

Purpose

Expresses K55M RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

K55M; MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET28 sf:mid.11

Plasmid

#191921

Purpose

Encodes a GFP design, sf:mid.11

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:mid.11

Tags

His Tag

Expression

Bacterial

Mutation

Q69M Y145F T167I L220V V224I

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:mid.7

Plasmid

#191933

Purpose

Encodes a GFP design, sf:mid.7

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:mid.7

Tags

His Tag

Expression

Bacterial

Mutation

S72T Y145F V224I

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 acid.6

Plasmid

#191912

Purpose

Encodes a GFP design, acid.6

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

acid.6

Tags

His Tag

Expression

Bacterial

Mutation

Q69L S72A Y145M T167V H181L

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:acid.3

Plasmid

#191926

Purpose

Encodes a GFP design, sf:acid.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:acid.3

Tags

His Tag

Expression

Bacterial

Mutation

T65S Q69L S72A T108V Y145M V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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