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pT2ADWpuro_2paRAF

Plasmid

#129653

Purpose

For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAIP

Plasmid

#74171

Purpose

Lentiviral vector for expression of heterologous genes

Depositor

Jeremy Luban

Article

Pertel et al Nature. 2011 Apr 21;472(7343):361-5. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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CMV500 A-C/EBP

Plasmid

#33352

Depositor

Charles Vinson

Article

Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

Insert

C/EBP (Cebpa Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
hBACCS2

Plasmid

#67625

Purpose

Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

hBACCS2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pFX-iRFP-Rab5

Plasmid

#174456

Purpose

Express iRFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with early endosome-targeting sequence

Tags

small GTPase (Rab5)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSW454 - pUFO

Plasmid

#115978

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pUFO

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG

Plasmid

#155131

Purpose

Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

His

Expression

Bacterial

Mutation

insert codes for GlpG residues 87-276

Promoter

T7

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIH-HA NEDD4L

Plasmid

#27028

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pSN23

Plasmid

#100108

Purpose

expresses a chimeric SOX2-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-SOX2 fused to a 'medium' VP16 domain (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-G9adSET

Plasmid

#41722

Purpose

Retroviral expression of human G9a mutant in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9adset (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCI syn iGluSnFR

Plasmid

#106123

Purpose

Indicator for glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR

Tags

Myc and PDGFR

Expression

Mammalian

Promoter

synapsin

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mAmetrine1.1-WPRE

Plasmid

#193340

Purpose

AAV-mediated expression of mAmetrine1.1 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mAmetrine1.1

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN HA

Plasmid

#188645

Purpose

This is the Lazarou lab's standard retrovirus vector backbone for expression of HA-tag fusions onto the N-terminus of cloned genes

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Type

Empty backbone

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFX-iRFP-Rab7

Plasmid

#174459

Purpose

Express iRFP with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with late endosome-targeting sequence

Tags

small GTPase (Rab7)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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dABE

Plasmid

#124447

Purpose

Expresses dead Cas9 (dCas9)-ABE7.10

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

dABE7.10

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SaCas9-BlastR

Plasmid

#220966

Purpose

Expresses human codon-optimized SaCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SaCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Sec61β

Plasmid

#174466

Purpose

Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with endoplasmic reticulum-targeting sequence

Tags

Sec61 subunit beta

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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AWP-031

Plasmid

#213967

Purpose

Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.

Depositor

Alexander Westermann

Article

Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

Insert

Cas12a (cas12a Segatella bryantii, Synthetic)

Tags

3XFLAG

Expression

Bacterial

Mutation

D875A to inactivate target nuclease activity

Promoter

PcfxA, IPTG inducible

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Article

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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GOMO-GFP

Plasmid

#60975

Purpose

Rapid selection of recombinant marker-free fluorescent rodent malaria parasites

Depositor

Olivier Silvie

Article

Manzoni et al Sci Rep. 2014 Apr 23;4:4760. doi: 10.103

Inserts

GFP
mCherry
hDHFR-yFCU

Use

Plasmodium gene targeting

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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