We narrowed to 18,496 results for: Met

1. pAc5.1B-lambdaN-HA-DmXRN1_A

   Plasmid

   #145879

   Purpose

   Insect Expression of DmXRN1

   Depositor

   Elisa Izaurralde

   Article

   Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

   Insert

   DmXRN1 (pcm Fly)
   

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA

   Plasmid

   #170768

   Purpose

   Methylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coli

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Inserts

   M.XhoI
   M.MunI
   M.Ocy1ORF8430P
   

   Expression

   Bacterial

   Promoter

   rhamnose

   Available Since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK617

   Plasmid

   #179297

   Purpose

   Expresses dCas9 fused to VP64

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

   Insert

   dCas9-VP64
   

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   CAG

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF-pntAB

   Plasmid

   #158609

   Purpose

   Low phosphate induction of pntAB

   Depositor

   Michael Lynch

   Article

   Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

   Insert

   pntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)
   

   Expression

   Bacterial

   Promoter

   Insulated ugpBp from E. coli

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCm N-term SMT3 NikJC199U

   Plasmid

   #174210

   Purpose

   Expression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Tags

   8xHis, SMT3, and TEV

   Expression

   Bacterial

   Mutation

   C199U

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCm C-term SMT3 NikJC199U

   Plasmid

   #174362

   Purpose

   Expression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Tags

   8xHis, SMT3, and TEV

   Expression

   Bacterial

   Mutation

   C199U

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCm N-term MBP NikJC199U

   Plasmid

   #174364

   Purpose

   Expression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Tags

   8xHis, MBP, and TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

   Plasmid

   #176573

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Tags

   Avitag, Hexahistidine tag, and mEGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

   Plasmid

   #176577

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Tags

   Hexahistidine tag and mVenus

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)

    Plasmid

    #173462

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-I-mNeonGreen
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)

    Plasmid

    #173460

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS41K-mCherry-mNeonGreen (pJJF003)

    Plasmid

    #173459

    Purpose

    C-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)

    Plasmid

    #173458

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-sfGFP
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. C453-E18: attB4-polyhedrinp>-attB5

    Plasmid

    #162977

    Purpose

    Gateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. C453-E21: attB4-FerHp>-attB5

    Plasmid

    #162978

    Purpose

    Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    human Ferritin heavy chain promoter
    

    Use

    Synthetic Biology

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. TetO-FUW-FLAG-NLS-KRAB-MCS_N-term

    Plasmid

    #136888

    Purpose

    Doxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    KRAB repressor domain
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TetO-FUW-MCS-KRAB_C-term

    Plasmid

    #136889

    Purpose

    Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the KRAB repressor domain and a STOP codon

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    KRAB repressor domain
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPRv2bleo-Nectin-2

    Plasmid

    #170295

    Purpose

    A knock-out vector for the mouse Nectin-2

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    A gRNA targeting the mouse Nectin-2 gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiCRISPRv2bleo-Necl-5

    Plasmid

    #170294

    Purpose

    A knock-out vector for the mouse Necl-5

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    A gRNA targeting the mouse Necl-5 gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJC8

    Plasmid

    #170757

    Purpose

    Co-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.

    Depositor

    Roberto Dominguez

    Article

    Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi

    Type

    Empty backbone

    Tags

    site 1- V5 tag and site 2- FLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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