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Plasmid

#40232

Depositor

Wolfhard Almers

Article

An et al Science. 2004 Nov 5;306(5698):1042-6.

Insert

Snap25b (SNAP25 Human)

Tags

CFP and Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA 3.1 Flag-rat-PTP1B

Plasmid

#35988

Depositor

Anna Huttenlocher

Article

Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

Insert

protein tyrosine phosphatase 1B (Ptpn1 Rat)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pBCN27-Pmyo-2::GFP::unc-54_3'UTR

Plasmid

#26347

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

Insert

Pmyo-2::GFP::unc-54_3'UTR

Expression

Worm

Available Since

Dec. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pMIR-CDH1 wtUTR

Plasmid

#25038

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

CDH1 3'UTR (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
hBax S184E C3-EGFP

Plasmid

#19743

Depositor

Richard Youle

Article

Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

Insert

Bax S184E (BAX Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Serine 184 to Glutamate

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP C1-WW45

Plasmid

#19052

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

SALVADOR 1 (SAV1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pRK DPC4 deltaC Flag

Plasmid

#12628

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

DPC4 delta C (SMAD4 Human)

Tags

flag

Expression

Mammalian

Mutation

C-terminal truncated DPC4 (aa 1-511)

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pBabe hygro myc p110-CAAX

Plasmid

#12591

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

p110-CAAX (PIK3CA Bovine)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-nuclear-EKAR4

Plasmid

#174439

Purpose

Nuclear-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

nuclear-EKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1.Gαi3-LgB91

Plasmid

#134359

Purpose

Nanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.

Depositor

Julien Hanson

Article

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

Gαi3-LgB91 (GNAI3 Human)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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Cherry-Fibrillarin-C1

Plasmid

#134539

Purpose

Expresses Cherry-Fibrillarin fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Fibrillarin (FBL Human)

Tags

Cherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-FBXL11

Plasmid

#126543

Purpose

Expresses GFP-tagged FBXL11 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

FBXL11 (KDM2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

LTR

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN K577M

Plasmid

#125790

Purpose

constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN K577M (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

K577M (helicase-dead)

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN E84A

Plasmid

#125789

Purpose

constitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN E84A (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

E84A (exonuclease-dead)

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGrin1

Plasmid

#124852

Purpose

Mutagenesis of Grin1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin1 (Grin1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-ch-TOG-His6

Plasmid

#69108

Purpose

For bacterial expression of recombinant human ch-TOG tagged with MBP and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)

Tags

His6 and MBP

Expression

Bacterial

Available Since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-KIAA1267

Plasmid

#29758

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

KIAA1267 (KANSL1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN alpha

Plasmid

#40928

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-SypHluorin 2x (S2x)

Plasmid

#37004

Depositor

Stephen Heinemann, Yongling Zhu

Article

Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

Insert

SypHluorin 2x (Syp Rat)

Tags

HA

Expression

Mammalian

Mutation

Two copies of pHluorin inserted at site 184-185

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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ChR2-YFP-NavII-III

Plasmid

#26057

Depositor

Matthew Grubb

Article

Grubb et al PLoS One. 2010 . 5(10):e13761.

Insert

NavII-III

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 15, 2010

Availability

Academic Institutions and Nonprofits only