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Showing: 9721 - 9740 of 79196 results

  1. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid
    #140082
    Purpose
    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter
    Depositor
    Keith Joung
    Insert
    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    Use
    In vitro transcription (mrna synthesis)
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140082-plasmid-map-sequence-id-292704

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  6. pBRα-β flap (831-1057)

    Plasmid
    #53734
    Purpose
    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.
    Depositor
    Ann Hochschild
    Publication
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    alpha NTD-Beta flap fusion
    Expression
    Bacterial
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53734-plasmid-map-sequence-id-83920

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  14. pB-CuR-hSOD1

    Plasmid
    #232477
    Purpose
    The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Publication
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Promoter
    CMV-CuO
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232477-plasmid-map-sequence-id-471678

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  15. pcDNA3.1-dCas9-dMQ1-EGFP

    Plasmid
    #89637
    Purpose
    Targeted CpG methylation
    Depositor
    Margaret Goodell
    Publication
    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    site-specific DNA-methyltransferase SssI
    Use
    CRISPR
    Tags
    3*FLAG, 6*His, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    C141S, TGC-->TCC; S317A, AGC-->GCC
    Promoter
    CMV
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89637-plasmid-map-sequence-id-177705

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  16. Dyn1(K44A)-mRFP

    Plasmid
    #55795
    Purpose
    Dominant negative Dynamin 1 mutant tagged with mRFP
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dynamin-1(K44A)-mRFP (Dnm1 Rat, Discosoma sp.)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    Dynamin-1(K44A)-mRFP was produced from Dynamin-1(…
    Promoter
    CMV
    Available since
    Sept. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55795-plasmid-map-sequence-id-89565

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  18. pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

    Plasmid
    #203350
    Purpose
    Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    Nano Luciferase
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Promoter
    T7
    Available since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203350-plasmid-map-sequence-id-399148

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Showing: 9721 - 9740 of 79196 results