We narrowed to 13,211 results for: CAR

1. pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227904

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227905

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   COPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pInducer10b-HA-KRAS G12V CO SR

   Plasmid

   #164928

   Purpose

   Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequence

   Depositor

   Ji Luo

   Article

   Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

   Insert

   KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Mutation

   Glycine 12 changed to valine; 9 Valine codons are…

   Promoter

   Ubc promoter

   Available Since

   May 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI

   Plasmid

   #126480

   Purpose

   Expresses Cas9-GSAlinker-TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   Cas9
   GSA linker
   TdT (DNTT Human)
   

   Expression

   Mammalian

   Mutation

   Changed codons to reduce repetitive sequences.

   Promoter

   CMV

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1α1.1-ChrimsonR-tdTomato

   Plasmid

   #122064

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1a（1.1kb short version)

   Available Since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CaMKII-ChrimsonR-tdTomato

   Plasmid

   #99231

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   CaMKII

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdest N-FLAG METTL1-WT puro

   Plasmid

   #223055

   Purpose

   METTL1-WT gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   METTL1-WT (METTL1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdest METTL1-CM Neo

   Plasmid

   #223061

   Purpose

   METTL1-CM gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   METTL1-CM (METTL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   160-163 (LFPD>AFPA)

   Promoter

   CMV

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

   Plasmid

   #113163

   Purpose

   A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS1951

    Plasmid

    #105868

    Purpose

    High copy number plasmid containing Hspa1a (synonym Hsp68) MiniPromoter insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Hspa1a (synonym Hsp68) MiniPromoter (HSPA1A, HSPA1A Human)
    

    Promoter

    Hsp68 MiniPromoter

    Available Since

    Feb. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAAV-Syn-Chronos-tdTomato

    Plasmid

    #62726

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV8

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    human synapsin promoter

    Available Since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5

    Plasmid

    #170995

    Purpose

    HiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    AKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ef1-alpha

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiCMVblast_SLC38A2_wt

    Plasmid

    #156180

    Purpose

    CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA se…

    Promoter

    CMV

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMS73

    Plasmid

    #110629

    Purpose

    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating

    Depositor

    Regine Kahmann

    Article

    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d

    Inserts

    short U6 promoter
    cas9
    tnos terminator
    

    Use

    CRISPR; Self-replicating in ustilago maydis, conf…

    Tags

    N-terminal NLS, C-terminal HA-tag +NLS

    Expression

    Bacterial

    Mutation

    Streptococcus pyogenes gene codon-optimized for U…

    Promoter

    U. maydis hsp70 promoter (Kronstad and Leong, 198…

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgAPC(A)

    Plasmid

    #85557

    Purpose

    Expresses sgRNA targeting human APC around codon I1417

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    APC, WNT signaling pathway regulator (APC Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pET30-2-PHGDH

    Plasmid

    #83907

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    Phosphoglycerate dehydrogenase (PHGDH Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. sgAPC(B)

    Plasmid

    #85558

    Purpose

    Expresses sgRNA targeting human APC around codon I1417

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    APC, WNT signaling pathway regulator (APC Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

18. AAV phSyn1(S)-FlpO-bGHpA

    Plasmid

    #51669

    Purpose

    Can be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter.

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV Retrograde

    Insert

    FlpO recombinase gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-ACE2-his expression plasmid

    Plasmid

    #158089

    Purpose

    Expresses ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    ACE2 (ACE2 Human)
    

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX6P1-hcGAS(157-522)

    Plasmid

    #108676

    Purpose

    For expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

    Insert

    cGAS amino acid 157-522 (CGAS Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acid 157-522

    Promoter

    tac

    Available Since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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