We narrowed to 165,750 results for: HTT

1. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

   Plasmid

   #217306

   Purpose

   sgRNA mcherry + Puromycin

   Depositor

   Luke Gilbert

   Insert

   pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
   

   Use

   CRISPR

   Promoter

   U6, Ef1alpha

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1-IRES eGFP

   Plasmid

   #128059

   Purpose

   Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJRD215

   Plasmid

   #167555

   Purpose

   Backbone plasmid to pRMA154

   Depositor

   Renato Morona

   Article

   Morona et al J Bacteriol. 1994 Feb;176(3):733-47. doi

   Type

   Empty backbone

   Use

   Broad host range mobilisable

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-fDIO-mCherry-WPREpA

   Plasmid

   #177328

   Purpose

   Control vector for Flp-dependent DREADD constructs

   Depositor

   Ulrik Gether

   Insert

   mCherry
   

   Use

   AAV; Flp-frt

   Expression

   Mammalian

   Promoter

   Human Synapsin-1

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a-mito-dsRED2

   Plasmid

   #174541

   Purpose

   mitochondria targeted dsRED expressed off of EF1a promoter

   Depositor

   Thomas Schwarz

   Insert

   Mito
   

   Use

   Lentiviral

   Tags

   dsRED

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTCF48 HLA-A*11:01

   Plasmid

   #180449

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   HLA-A*11:01
   

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Available Since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWPI

   Plasmid

   #12254

   Purpose

   2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.

   Depositor

   Didier Trono

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   EMCV IRES-EGFP cassette

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJA54 HLA-A*03:01

   Plasmid

   #180451

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   HLA-A*03:01
   

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

   Plasmid

   #107055

   Purpose

   Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

   Depositor

   Alex Hewitt

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti.Cas9.BFP.Blast

    Plasmid

    #196714

    Purpose

    WT SpCas9 with BSD-TagBFP for selection and sorting

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-Paxillin

    Plasmid

    #80029

    Purpose

    Mammalian expression of mouse Paxillin

    Depositor

    Chinten James Lim

    Insert

    Paxillin (Pxn Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. R777-E172 Hs.PIK3R1-nostop

    Plasmid

    #70456

    Purpose

    Gateway ORF clone of human PIK3R1 [NM_181523.2] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-3'3xFlag

    Plasmid

    #208616

    Purpose

    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOpen-DNA Topoisomerase 1B Vaccinia Virus

    Plasmid

    #165510

    Purpose

    Used in TOPO cloning. Recognizes the DNA sequence 5'-(C/T)CCTT-3' and digests double stranded DNA at this sequence.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    DNA Topoisomerase 1B Vaccinia Virus
    

    Use

    Synthetic Biology

    Available Since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69545

    Purpose

    Tet-On system expressing GFP tagged with 2x nuclear localization signals.

    Depositor

    Charles Gersbach

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJV53

    Plasmid

    #26904

    Depositor

    Graham Hatfull

    Article

    van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

    Insert

    Che9c genes 60-61
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFAP-Flex-MCS-WPRE-SV40 - GCH32

    Plasmid

    #230830

    Purpose

    AAV cloning vector for CRE-dependent expression under the GFAP promoter.

    Depositor

    Kimberly Ritola

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; Cloning vector

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCLIP-tag (m) Vector

    Plasmid

    #101136

    Purpose

    Expression of protein of interest with CLIP-Tag in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Type

    Empty backbone

    Tags

    CLIP-tag (CLIP10)

    Expression

    Mammalian

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLIX403-EYFP-ccdB-Hygro

    Plasmid

    #158555

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. KP.3

    Plasmid

    #233340

    Purpose

    expressing SARS-CoV-2 KP.3 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (KP.3) (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    F456L, Q493E, V1104L on the basis of JN.1

    Promoter

    CMV

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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