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pETURALEU

Plasmid

#223207

Purpose

Plasmid for selection/counterslection in budding yeast, containing URA3 and LEU2 markers

Depositor

Sahand Jamal Rahi

Article

Stojkovic et al G3 (Bethesda). 2024 Dec 17:jkae297. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b-C-MBP

Plasmid

#214304

Purpose

Provide a bacterial expression vector that encodes C-terminus MBP tag

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Type

Empty backbone

Expression

Bacterial

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBH102

Plasmid

#215773

Purpose

NBU2 backbone with LacI(TAN) and P(tta)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(TAN)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(tta)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH103

Plasmid

#215774

Purpose

NBU2 backbone with LacI(KSL) and P(agg)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(KSL)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(agg)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH104

Plasmid

#215775

Purpose

NBU2 backbone with LacI(HQN) and P(ttg)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(HQN)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(ttg)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH105

Plasmid

#215776

Purpose

NBU2 backbone with LacI(GKR) and P(gac)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(GKR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(gac)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH116

Plasmid

#215779

Purpose

NBU2 backbone with anti-RbsR(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

anti-RbsR(YQR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(O1)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH501

Plasmid

#215780

Purpose

NBU1 backbone with constitutive dCas9

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Insert

dCas9

Use

Synthetic Biology

Expression

Bacterial

Promoter

P1

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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RT-1

Plasmid

#220434

Purpose

Base construct used to create PUP2/3HA fusion proteins in S. cerevisiae. (pBSII/PUP2/3HA/URA3)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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T8-pAc5.1 lambdaN HA EcoRV

Plasmid

#206492

Purpose

Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Type

Empty backbone

Expression

Insect

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMYT082

Plasmid

#198864

Purpose

Markerless yeast integration Vector at Int.8 locus to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-Exo-Blast-BFP

Plasmid

#196721

Purpose

Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

Depositor

Bernhard Schmierer

Insert

Cas9-Exo1-T2A-BSD-TagBFP

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

EF1a

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMYT076

Plasmid

#180729

Purpose

Markerless yeast integration Vector at Int.2 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT083

Plasmid

#180736

Purpose

Markerless yeast integration Vector at Int.9 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT084

Plasmid

#180737

Purpose

Markerless yeast integration Vector at Int.10 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT081

Plasmid

#180734

Purpose

Markerless yeast integration Vector at Int.7 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT080

Plasmid

#180733

Purpose

Markerless yeast integration Vector at Int.6 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT079

Plasmid

#180732

Purpose

Markerless yeast integration Vector at Int.5 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT078

Plasmid

#180731

Purpose

Markerless yeast integration Vector at Int.4 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT077

Plasmid

#180730

Purpose

Markerless yeast integration Vector at Int.3 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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