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Plasmid#115235PurposeLentiviral transfer plasmid for expression of mTagBFP2 before Flp recombination and mCherry after itDepositorInsertmTagBFP2 and mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
B9L-BirA*
Plasmid#109146PurposeTo generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cellsDepositorInsertB9L (Bcl9l Mouse)
UseTagsBirA(R118G) and FlagExpressionMammalianMutationPromoterCMV/TetO2Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/SH/GW-Mst1
Plasmid#84299PurposeExpresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.DepositorInsertMst1 (STK4 Human)
UseTagsHA, SBPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
EW411 (UTRN-43-5bp spacer-UTRN-14)-H2B-GFP
Plasmid#236148PurposeFluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the UTRN promoter region encompassing 14 to 67 bp upstream of its transcriptional start siteDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterE1b minimal promoter with the UTRN promoter regionAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
JPZF1 CMV-TO-SCN1A-377(G2E, A104K, S109I)-deImmLink-NZF
Plasmid#236153PurposeExpress mutated SCN1A-14 zinc finger array (G2E, A104K, S109I) attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-377-deImmLink-NZF
UseTagsExpressionMammalianMutationG2E, A104K, S109I in SCN1A-377PromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ388 (IVT) CMV-TO-T7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3
Plasmid#233424PurposeIVT template for LIDAR reporter mRNA; EGFP as marker, Cre recombinase as outputDepositorInsertT7-EGFP-SGc(stem-1(3xMS2))cSG-Cre-hybridUTR3
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ034 CMV-TO-CCL20
Plasmid#233416Purposeplasmid expressing secreted soluble CCL20DepositorInsertCCL20 (CCL20 Human)
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ362 EF1a-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export
Plasmid#233381PurposeOptimized VEGF-sensing eLIDAR with VEGFR2 ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequenceDepositorInsertVEGFR2(ecto+TM)-tdMCP
UseTagsHAExpressionMammalianMutationWTPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ363 EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export
Plasmid#233382PurposeOptimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequenceDepositorInsertVEGFR1(ecto+TM)-ADAR2dd
UseTagsFLAGExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ375 EF1a-IL2Rbsp-3xFLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export
Plasmid#233386PurposeOptimized IL-2-sensing eLIDAR with IL-2 beta chain ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequenceDepositorInsertIL2Rb(ecto+TM)-ADAR2dd
UseTagsFLAGExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ374 EF1a-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export
Plasmid#233389PurposeOptimized IL-2-sensing eLIDAR with IL-2 gamma chain ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequenceDepositorInsertIL2Rg(ecto+TM)-tdMCP
UseTagsHAExpressionMammalianMutationWTPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-N
Plasmid#210270PurposeN-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.DepositorTypeEmpty backboneUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP
Plasmid#205604PurposeTet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertionDepositorInsertγ-tubulinΔGTP-RNAi-resistant-GFP
UseTagsGFPExpressionMammalianMutationG2228A, C2231T, C2237T, G2240A, N229APromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBA-Con/Fon-EYFP-WPRE
Plasmid#186752PurposeAAV-mediated Cre and Flp-dependent expression of EYFP under CBA promoterDepositorInsertEYFP
UseAAVTagsExpressionMutationPromoterCBAAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-pAce-Kv2.1PR
Plasmid#195535PurposeFlp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targetedDepositorInsertpAce-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 78K, 81D, 92N, 178F; SY linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-AID-BubR1
Plasmid#47330Purposefor plasmid integration using the Flp-In system. AID at N-terminalDepositorInsertBUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
UseTagsAID and GFPExpressionMammalianMutationsiRNA resistantPromoterAvailable sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer
Plasmid#90013PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-DicerDepositorInsertsCBirA*-Ago2
NBirA*-Dicer
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDC-UAS-nBitbow1.0(miniW)
Plasmid#138329PurposeDrosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.DepositorInsertsmAmetrine
tdKatushka2
mNeonGreen
mTFP1
mKusabira-Orange2
UseTagshuman H2BExpressionInsectMutationPromoter10xUASAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP
Plasmid#90015PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFPDepositorInsertsCBirA*-Ago2
NBirA*-GFP
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA
Plasmid#97012PurposeRosa26 targeting vector for intersectional Cre and FLP responsive cassetteDepositorTypeEmpty backboneUseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only