Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

3,646 results

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pTwist Amp_d5-HT7R-sfGFP11-HA-CRISPR-HDR-donor

Plasmid

#221819

Purpose

Donor plasmid for inserting GFP11-HA to the end of Drosophila 5-HT7R coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

GFP11-HA donor with homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA-d5-HT1AR

Plasmid

#221820

Purpose

Plasmid to express gRNA (gaccagtccactaccgcagc) for editing at the end of Drosophila 5-HT1AR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT1AR gRNA (5-HT1A Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA1-d5-HT1BR

Plasmid

#221821

Purpose

Plasmid to express gRNA1 (gaaaatttgatttcaactga) for editing at the end of Drosophila 5-HT1BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT1BR gRNA1 (5-HT1B Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA2-5-HT1BR

Plasmid

#221822

Purpose

Plasmid to express gRNA2 (aatttcgacgggccttcaag) for editing at the end of Drosophila 5-HT1BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT1BR gRNA2 (5-HT1B Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA1-d5-HT2BR

Plasmid

#221827

Purpose

Plasmid to express gRNA1 (ttcagtttgcccggtttaac) for editing at the end of Drosophila 5-HT2BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT2BR gRNA1 (CG42796 Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA2-d5-HT2BR

Plasmid

#221828

Purpose

Plasmid to express gRNA2 (aggcactcgtgctcgaatag) for editing at the end of Drosophila 5-HT2BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT2BR gRNA2 (CG42796 Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA-d5-HT7R

Plasmid

#221829

Purpose

Plasmid to express gRNA (ggcgagggagagctttctct) for editing at the end of Drosophila 5-HT1AR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT7R gRNA (5-HT7 Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA1-for-dSERT

Plasmid

#221830

Purpose

Plasmid to express gRNA1 (cgaaatctgcgctctacttg) for editing at the beginning of Drosophila SERT coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

dSERT gRNA1 (SerT Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA2-for-dSERT

Plasmid

#221831

Purpose

Plasmid to express gRNA2 (gggattcgagcggcccgtcg) for editing at the beginning of Drosophila SERT coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

dSERT gRNA2 (SerT Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT1AR-7xsfGFP11-HA-HDR-donor

Plasmid

#221832

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1AR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT1AR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2BR-7xsfGFP11-HA-HDR-donor

Plasmid

#221833

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2BR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_2xFlag-dSERT-HDR-donor-HDR

Plasmid

#221835

Purpose

Donor plasmid for inserting 2xFLAG at the beginning of Drosophila SERT coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

2xFLAG with dSERT homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pACUH 5-HT7R-7xGFP11-HA

Plasmid

#221836

Purpose

Plasmid for generating 10xUAS-5-HT7R-7xGFP11-HA transgenic flies with attP/attB integration. It carries the attB sequence.

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

5-HT7R (5-HT7 Fly)

Tags

7xGFP11-HA

Expression

Insect

Promoter

hsp70

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT1BR-7xsfGFP11-HA-HDR-donor

Plasmid

#221837

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT1BR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist Amp_d5-HT2AR(isoformD)-7xsfGFP11-HA-HDR-donor

Plasmid

#221838

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoform D coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2AR (isoform D) homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2AR(isoformBFH)-7xsfGFP11-HA-HDR-donor

Plasmid

#221839

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2AR (isoforms BFH) homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pACUH 5-HT2BR-7xGFP11-HA

Plasmid

#221840

Purpose

Plasmid for generating 10xUAS-5-HT2BR-7xGFP11-HA transgenic flies with attP/attB integration. It carries the attB sequence.

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

5-HT2BR (CG42796 Fly)

Tags

7xGFP11-HA

Expression

Insect

Promoter

hsp70

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-spatzle-HA

Plasmid

#240235

Purpose

Expression of spatzle-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

spatzle (spz Fly)

Tags

HA-spGFP11

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-hhN-HA

Plasmid

#240236

Purpose

Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

hedgehog N-terminal fragment (hh Fly)

Tags

HA-spGFP11

Expression

Insect

Mutation

aa 1-257

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-CG6867-sfGFP

Plasmid

#240237

Purpose

Expression of CG6867-sfGFP under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

CG6867 (CG6867 Fly)

Tags

sfGFP

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 961 - 980 of 3646 results

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