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Plasmid#183157PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only
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pYL322d1/N-Vn
Plasmid#183153PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGreen_dualluc_ORF_sensor
Plasmid#55207PurposeDual-luciferase reporter for microRNA targeting in plantsDepositorInsertsRenilla luciferase
Firefly luciferase
UseLuciferase; Plant transient expressionTagsExpressionMutationPromoterAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lifeact_HaloTag9
Plasmid#175545PurposeExpression of HaloTag9 at the actin filaments of mammalian cellsDepositorInsertLifeact-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_ALFA-tag-SNAP
Plasmid#136628PurposePlasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusionDepositorInsertEGFP-NbALFA
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIM24 KanR
Plasmid#67583PurposePlant Gene Expression VectorDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterM24Available sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Vc-C
Plasmid#183158PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYL322d1/Vn-C
Plasmid#183154PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoNanobody
Plasmid#136622PurposePlasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusionDepositorInsertNTOM20-SNAP-Tag-EnhancerNb
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV-EGFP-2A-mNeonGreen
Plasmid#171599PurposeGreen lentiviral vector plasmid for pseudovirus experimentsDepositorInsertsEGFP
mNeonGreen
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTX171
Plasmid#89258PurposeExpress STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe
Plasmid#183161PurposeConstruct for transient expression of cell nucleus-, cytoplasm-targeted mCherry in plants.DepositorInsertmCherry
UseTagsExpressionPlantMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB01
Plasmid#111957PurposeExpresses His10-RFP-Halo; construct generated for pull-down and localization experimentsDepositorInsertRFP
UseTagsHaloTagExpressionBacterialMutationPromoterT7Available sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTX179
Plasmid#89261PurposeExpress STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-RDSmCherry1
Plasmid#89987PurposeRed-Shifted variants of red fluorescent protein mCherryDepositorInsertRDSmCherry1
UseTags6xHis-T7-XpressExpressionBacterialMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTX172
Plasmid#89259PurposeExpress STU (Single Transcriptional Unit) Cas9 with Maize Ubiquitin1 promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICSL50016
Plasmid#50315DepositorInsertCDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
UsePlant expressionTagsExpressionMutationCodon-optimised for A. thaliana. BsaI/BbsI sites …PromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTX176
Plasmid#89260PurposeExpress estrodial-inducible STU (Single Transcriptional Unit) Cas9 in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynSNAP IRES EGFP
Plasmid#136625PurposePlasmid encoding outer inner membrane localization of SNAP-Tag and EGFPDepositorInsertLyn11-SNAP-Tag (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSi
Plasmid#67884PurposeDerivative of pSiM24 plant expression vector without the M24 promoterDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTX168
Plasmid#89257PurposeExpress STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH42222
Plasmid#50328DepositorInsertCDS, phosphinothricin acetyl transferase, (S. hygroscopicus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutation; See De…PromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS313-PRP8
Plasmid#89966Purposecomplements prp8 genomic deletionDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationnonePromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUbiSXR
Plasmid#64403PurposeUbiquitin promoter driving synthetic xylanase (sXynA) gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.DepositorInsertxylanase
UseTagsExpressionPlantMutationcodon optimized for plant expressionPromoterPlant ubiquitin (Ubi-1) promoter (with untranslat…Available sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS314-PRP16
Plasmid#89971PurposePrp16 in a trp backbone complements prp16 genomic deletionDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationnonePromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB605-GBSSI
Plasmid#213867PurposeBinary vector for the expression of GBBSI fused to the sGFP in plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertGRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB560-GBSSI
Plasmid#213866PurposeBinary vector for the expression of GBBSI fused to the tagRFP in plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertGRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI
Plasmid#192113PurposeExpresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-NTD-BA.4/5 (S )
UseTagsAVI, HRV3C, and scFcExpressionMammalianMutationT19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …PromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
XE5 Xwnt-5A Hind RNA
Plasmid#16872DepositorInsertXwnt-5A Hind RNA (wnt5a.S Frog)
UseXenopus expressionTagsExpressionMutationStarts with AA 14 (see comments)PromoterAvailable sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH-ePPE
Plasmid#183097PurposeFor plant prime editing in rice plantsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9PromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only