We narrowed to 6,371 results for: siae
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Plasmid#67541PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only
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L284F Cus2p
Plasmid#73059PurposeS. cerevisiae Cus2p gene with L284F MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
Tags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationLeucine 284 to PhenylalaninePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
D282N Cus2p
Plasmid#73060PurposeS. cerevisiae Cus2p gene with D282N MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
Tags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationAsp 282 to AsnPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNZ18-CAH
Plasmid#203159PurposeReplicates in E. coli, S. cerevisiae, and A. laidlawiiDepositorInsertYeast centromere, autonomously replicating sequence, histidine auxotrophic marker
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZH82 pET28a-Rai1
Plasmid#231651PurposeBacterial expression vector expressing yeast S. cerevisiae rai1, no tagDepositorInsertrai1 (RAI1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH23 Dual G-less containing ySSA1 polyA
Plasmid#231647Purposeyeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassetteDepositorInsertSSA1 polyA termination region
UseSynthetic BiologyAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH20 Dual G-less containing yCYC1 polyA
Plasmid#231646Purposeyeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassetteDepositorInsertCYC1 polyA termination region
UseSynthetic BiologyAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBF3060
Plasmid#26853PurposeExpression of yeast codon-optimized CreA recombinase from the GAL1 promoterDepositorInsertCreA recombinase
UseCre/LoxExpressionYeastMutationN/APromoterGAL1Available SinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pZH61 pLIB-8His3C-spt4
Plasmid#231649Purposeexpress S.cerevisiae spt4 in insect cellsDepositorAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS423-GPD-wrmScarlet1-10-CYC1 terminator
Plasmid#158584PurposeExpresses wrmScarlet1-10 in S. cerevisiaeDepositorInsertwrmScarlet1-10
ExpressionYeastPromoterGPDAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
RCP83
Plasmid#163466PurposeBackbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFPDepositorInsertsPromoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP
ExpressionBacterial and YeastAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRG705_lexO-AsiSI_LexA-TF_LEU2MX
Plasmid#154817PurposeInducible expression of AsiSI endonuclease in S. cerevisiaeDepositorInsertsAsiSI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILFPQ13BE5
Plasmid#223836PurposeExpressing a cytosolic cAMP sensor (mScarletI3-ApacH-StayGold2) in Saccharomyces cerevisiaeDepositorInsertpTEF1>mScarletI3>ApacH>StayGold2
ExpressionYeastAvailable SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRG706_lexO-SbfI_LexA-TF_LEU2MX
Plasmid#154818PurposeInducible expression of SbfI endonuclease in S. cerevisiaeDepositorInsertsSbfI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
12URA-B-ScTop2∆CTD
Plasmid#214924PurposeYeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)DepositorInserttopoisomerase II
Tags6x His-TEVExpressionYeastMutationDeleted amino acids 1178-1428PromoterGal 1/10Available SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRG659_lexO-FseI_LexA-TF_LEU2MX
Plasmid#154816PurposeInducible expression of FseI endonuclease in S. cerevisiaeDepositorInsertsFseI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG645_lexO-AscI_LexA-TF_LEU2MX
Plasmid#154813PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRG714_lexO-AscI_LexA-TF_URA3MX
Plasmid#154822PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
SIV URA PCP-NLS-GFPEnvy
Plasmid#189941PurposeSingle-integration vector for inserting PCP-NLS-GFPEnvy at the URA3 locus in Saccharomyces cerevisiaeDepositorInsertPCP-NLS-GFPEnvy
ExpressionMammalianAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4
Plasmid#87733PurposeBacterial expression of full-length S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEVExpressionBacterialPromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-ScVrg4
Plasmid#102334PurposeExpresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHES609 [prNOp7-PBAC]
Plasmid#103017PurposeA S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.DepositorInsertbPAC
ExpressionYeastAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ4
Plasmid#83559PurposeUsed to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.DepositorInsertGAL7 promoter-yEGFP
ExpressionYeastPromoterGAL7Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dom34(full length)
Plasmid#19098DepositorAvailable SinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
iRFP713-FRB-Mss4-Kina
Plasmid#202744Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorInsertiRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)
ExpressionMammalianAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET5
Plasmid#107437PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET5 (MET5 Budding Yeast)
TagsV5-HisExpressionYeastMutationV81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …PromoterGAL1Available SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
JBEI-6833
Plasmid#84983PurposeExpression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.DepositorInsertNudB-PMD
ExpressionBacterialPromotertrc promoterAvailable SinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRG711_lexO-AscI_LexA-TF_HIS3MX
Plasmid#154820PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only