We narrowed to 30,903 results for: LIS;

1. pcmv3-IRG1-HA

   Plasmid

   #198181

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1)

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   HA tag is added to C-terminal of target protein

   Promoter

   CMV

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-dual-scafffold-2.0 (pBP49)

   Plasmid

   #218931

   Purpose

   The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Insert

   dual scaffolds for CRISPR
   

   Use

   CRISPR

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-dual-CRISPR-U6-H1(pBP43)

   Plasmid

   #218930

   Purpose

   The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   human U6 and H1

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-rtTA

   Plasmid

   #175274

   Purpose

   AAV vector mediating neuron-specific expression of rtTA

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   Reverse tetracycline-controlled transactivator (rtTA)
   

   Use

   AAV

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. YFVdelCME-mVenus

   Plasmid

   #179953

   Purpose

   pCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   YFV-17D lacking C, prM, E proteins but with mVenus
   

   Use

   Transneuronal tracing

   Promoter

   SP6

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-tTA

   Plasmid

   #175280

   Purpose

   AAV vector mediating neuron-specific expression of tTA

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   tTA advanced (improved tetracycline-controlled transactivator)
   

   Use

   AAV

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. YFVdelNS1-Cre

   Plasmid

   #179952

   Purpose

   pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with Cre

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   YFV-17D lacking nonstructural protein 1 (NS1), but with Cre
   

   Use

   Transneuronal tracing

   Promoter

   SP6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcmv3-IRG1-N152S-HA

   Plasmid

   #198182

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1) N152S

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The asparagine in IRG1 at position 152 mutates to…

   Promoter

   CMV

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YFVdelNS1-mCherry

   Plasmid

   #179951

   Purpose

   pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with mCherry

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   YFV-17D lacking nonstructural protein 1 (NS1) but with mCherry
   

   Use

   Transneuronal tracing

   Promoter

   SP6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. YFVdelNS1-mVenus

    Plasmid

    #179950

    Purpose

    pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with mVenus

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    YFV-17D lacking Nonstructural protein 1 (NS1) but with mVenus
    

    Use

    Transneuronal tracing

    Promoter

    SP6

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETDUET-GST AO7REwt

    Plasmid

    #213935

    Purpose

    GST tagged AO7 encoding aa 126-258 for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    AO7 cloned into the first multiple cloning site o…

    Promoter

    T7 promoter-1

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcmv3-IRG1-L272Q-HA

    Plasmid

    #198184

    Purpose

    Mammalian expression of HA-tagged human ACOD1 (IRG1) L272Q

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    ACOD1 (ACOD1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The leucine in IRG1 at position 272 mutates to gl…

    Promoter

    CMV

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. YFVdelCMENS1-Cre

    Plasmid

    #179954

    Purpose

    pCCI vector encoding YFV-17D lacking C, prM, E and NS1 proteins but with Cre

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    YFV-17D lacking C, M, E and NS1 proteins but with Cre
    

    Use

    Transneuronal tracing

    Promoter

    SP6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcmv3-STING-C88S-FLAG

    Plasmid

    #198187

    Purpose

    Mammalian expression of FLAG-tagged mouse STING1 C88S

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    TMEM173 (Sting1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcmv3-IRG1-H159Q-HA

    Plasmid

    #198183

    Purpose

    Mammalian expression of HA-tagged human ACOD1 (IRG1) H159Q

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    ACOD1 (ACOD1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The histidine in IRG1 at position 159 mutates to …

    Promoter

    CMV

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcmv3-IRG1-H277Y-HA

    Plasmid

    #198185

    Purpose

    Mammalian expression of HA-tagged human ACOD1 (IRG1) H277Y

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    ACOD1 (ACOD1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The histidine in IRG1 at position 277 mutates to …

    Promoter

    CMV

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid

    #184774

    Purpose

    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Mouse, Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETDUET-GST AO7RE Y165A

    Plasmid

    #139428

    Purpose

    GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed tyrosine 165 to an alanine

    Promoter

    T7 promoter-1

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETDUET-GST AO7RE P199A

    Plasmid

    #139427

    Purpose

    GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed proline 199 to an alanine

    Promoter

    T7 promoter-1

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETDUET-GST AO7RE I137A

    Plasmid

    #139319

    Purpose

    GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed isoleucine 137 to an alanine

    Promoter

    T7 promoter-1

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge