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PL-CRISPR.EFS.tRFP-gIfngr1-1

Plasmid

#166479

Purpose

Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Ifngr1 (Ifngr1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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PL-CRISPR.EFS.tRFP-gIfngr1-2

Plasmid

#166480

Purpose

Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Ifngr1 (Ifngr1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5AdeltaSIV GFP Nter

Plasmid

#145770

Purpose

expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

Depositor

Hugues Abriel

Article

Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-YAP-mEGFP

Plasmid

#166472

Purpose

HDR template for tagging of endogenous mouse YAP N-terminus with mEGFP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP-mEGFP HDR template(mouse Yap) (Yap1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAPS127A-deltaCC

Plasmid

#166459

Purpose

Expresses fusion of mEGFP and YAPS127A-deltaCC (delta298-359)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A-deltaCC (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-CRISPR-v2-gYap-1

Plasmid

#166481

Purpose

Expresses spCas9 and a gRNA targeting mouse Yap

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Yap (Yap1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A- L308E

Plasmid

#166462

Purpose

Expresses fusion of mEGFP and YAPS127A- L308E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A- L308E (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

YAPS127A- L308E

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A-deltasIDR

Plasmid

#166460

Purpose

Expresses fusion of mEGFP and YAPdeltasIDR (delta360-449)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A-deltasIDR (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A-deltasTAD

Plasmid

#166461

Purpose

Expresses fusion of mEGFP and YAPdeltasTAD (delta450-504)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A-deltasTAD (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry-coiled coil

Plasmid

#166448

Purpose

Expresses fusion of CRY2PHR with mCherry and YAP Coiled-coil domain

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, coiled coil(298-359) (CRY2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-CRISPR-V2-gYap-2

Plasmid

#166482

Purpose

Expresses spCas9 and a gRNA targeting mouse Yap

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Yap (Yap1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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Pet22b-mEGFP-YAP-N-IDR

Plasmid

#166440

Purpose

Bacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP-N-IDR(1-164) (YAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A- L325E

Plasmid

#166464

Purpose

Expresses fusion of mEGFP and YAPS127A- L325E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A- L325E (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

YAPS127A- L325E

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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Pet22b-mCherry-TEAD4-IDR

Plasmid

#166442

Purpose

Bacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, TEAD4-IDR (TEAD4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A- L318E

Plasmid

#166465

Purpose

Expresses fusion of mEGFP and YAPS127A- L318E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A- L318E (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

YAPS127A- L318E

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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Pet22b-mEGFP-YAP-C-IDR

Plasmid

#166441

Purpose

Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP-C-IDR(266-504) (YAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-neo-YAP-deltaCC

Plasmid

#166469

Purpose

Plasmid coding for YAP-deltaCC (delta298-359) and neomycin resistance in a lentiviral backbone.

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP-deltaCC, Neo (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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CCR5-SZ218a QTY

Plasmid

#162444

Purpose

Expression in E coli and perform ligand binding in solution

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-C chemokine receptor type 5 (CCR5 Human)

Use

Tags

his-tag

Expression

Bacterial

Mutation

All L, I, V, F in the transmembrane region mutate…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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CCR5-SZ190b QTY

Plasmid

#162445

Purpose

Expression in E coli and perform ligand binding in solution

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-C chemokine receptor type 5 (CCR5 Human)

Use

Tags

his-tag

Expression

Bacterial

Mutation

All L, I, V, F in the transmembrane region mutate…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4-ires-blast

Plasmid

#167829

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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