We narrowed to 3,244 results for: multiplex

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pcDNA3-luxOFP

Plasmid

#229950

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

Depositor

Andy Yeh

Publication

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxOFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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(attB) BRaf with MS2-circRNA

Plasmid

#226164

Purpose

Used to integrate HA-tagged BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines

Depositor

Dustin Maly

Publication

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Inserts

2xHA-BRaf (BRAF Human)
IRES-mCherry-P2A-PuroR
MS2-circRNA barcode

Tags

2xHA

Expression

Mammalian

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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ePPE-V223A

Plasmid

#205243

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Publication

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

SV40_NLS-nCas9-NC-MLV-SV40_NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ230-D156R (ttLbCas12a)

Plasmid

#195366

Purpose

A temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12a

Depositor

Yiping Qi

Publication

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

LbCas12a-D156R (ttLbCas12a)

Use

CRISPR; Gateway compatible lbcas12a-d156r (ttlbca…

Expression

Plant

Promoter

ZmUBI1

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-p300_Blast

Plasmid

#192654

Purpose

3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBYK01

Plasmid

#138656

Purpose

phiOZJ integrating Streptomyces expression vector lacking RDF

Depositor

Joshua Blodgett

Publication

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Expression

Bacterial

Promoter

PermE* variant sequence from pDA1652

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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p4xSMAD_RE

Plasmid

#118051

Purpose

GBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

4 copies the SMAD DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Expression

Mammalian

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-AKARev(T/A)

Plasmid

#118479

Purpose

Negative-control mutant for RAB-AKARev biosensor.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-AKARev(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI

Plasmid

#115220

Purpose

Expresses Pin3Cas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

Pin3Cas13

Tags

SUMO

Expression

Bacterial

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

Plasmid

#115210

Purpose

Expresses AspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

AspCas13

Tags

SUMO

Expression

Bacterial

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

Plasmid

#115207

Purpose

Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

BzoCas13

Tags

SUMO

Expression

Bacterial

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-OFF VIPER CAR (anti-CD19)-candidate (c+iii)

Plasmid

#192122

Purpose

Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(c+iii)

Depositor

Wilson Wong

Publication

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pEF1a-anti-CD19scFV_CD28_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry

Use

Lentiviral

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pWS3805

Plasmid

#185746

Purpose

Sub-array spacer 1/4

Depositor

Rodrigo Ledesma-Amaro

Publication

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 1/4

Use

CRISPR

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pWS3808

Plasmid

#185749

Purpose

Sub-array spacer 4/4

Depositor

Rodrigo Ledesma-Amaro

Publication

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 4/4

Use

CRISPR

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:GLuc:bgH

Plasmid

#135294

Purpose

Transcriptional unit for constitutive expression of GLuc in mammalian cells

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:GLuc:bGH

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pUHD-SB-mAID/Bla

Plasmid

#171680

Purpose

TRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (blasticidin)

Depositor

Randy Poon

Publication

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Type

Empty backbone

Tags

mAID

Expression

Mammalian

Promoter

TRE

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pUHD-SB-mAID/Hyg

Plasmid

#171679

Purpose

TRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (hygromycin)

Depositor

Randy Poon

Publication

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

mAID

Expression

Mammalian

Promoter

TRE

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pYZ673

Plasmid

#132953

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombe

Depositor

Jef Boeke

Publication

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

FnCas12a

Use

CRISPR and Synthetic Biology

Promoter

adh1

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-FCU1

Plasmid

#165882

Purpose

Basic 5-fluorocytosine sensitivity cassette in Alpha2, lacks physical marker or attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-TK

Plasmid

#165881

Purpose

Basic ganciclovir or acyclovir sensitvity cassette in Alpha2, lacks physical marker or attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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