We narrowed to 5,093 results for: puc

1. pUC18-mini-Tn7T-Plac-dCas9 (Plac)

   Plasmid

   #105235

   Purpose

   Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Bacterial

   Mutation

   D10A, H599A

   Available Since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

   Plasmid

   #133794

   Purpose

   U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   NmeCas9 and Nme2Cas9 sgRNA entry vector
   

   Expression

   Mammalian

   Mutation

   sgRNA architecture from Amrani et al. Genome Biol…

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-TgKI-MCS-eGFP-MCS-polyA

   Plasmid

   #174024

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   eGFP
   

   Use

   Zebrafish knock-in tagging

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC18-mini-Tn7T-Lac-dCas9 (Ptac)

   Plasmid

   #105234

   Purpose

   Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Bacterial

   Mutation

   D10A, H599A

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTR-346 pUC19-tNGFR-P2A-IL2RA N

   Plasmid

   #186065

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   IL2RA (IL2RA Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)

   Plasmid

   #215759

   Purpose

   HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)

   Depositor

   Dimitrios Wagner

   Article

   Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

   Insert

   CD247-truncCD19CAR-GSG (CD19, CD247 Human)
   

   Tags

   IgG1 CH3

   Expression

   Bacterial

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

   Plasmid

   #195503

   Purpose

   SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
   

   Use

   CRISPR; Donor vector

   Tags

   T2A-H2B-mCitrine

   Expression

   Mammalian

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-TgKI-MCS-p2A-tdTomato-polyA

   Plasmid

   #174027

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-tdTomato
   

   Use

   Zebrafish knock-in tagging

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-Top2A-5X Gly-Venus-T2A-Hygro

   Plasmid

   #211115

   Purpose

   For tagging the C-term of Top2A with Venus

   Depositor

   Job Dekker

   Article

   Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

   Inserts

   5' C-term Top2A homology arm (TOP2A Human)
   Venus
   T2A
   Blastocidin Resistance
   5' C-term Top2A homology arm (TOP2A Human)
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   n/a

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-TgKI-MCS-mScarlet-MCS-polyA

    Plasmid

    #174023

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    mScarlet
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

    Plasmid

    #114087

    Purpose

    U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for FnCas12a crRNAs
    

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTR-338 pUC19-tNGFR-P2A-LAG3 N

    Plasmid

    #186055

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    LAG3 (LAG3 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTR-339 pUC19-tNGFR-P2A-TIM3 N

    Plasmid

    #186077

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    TIM3 (HAVCR2 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Padh1-OsTIR1(WT)-NLS-TADH1-Sp.arg3+/pUC19*

    Plasmid

    #169352

    Purpose

    Expression of OsTIR1(WT) in S. pombe from adh1 promoter, integrated at arg3-D4 locus

    Depositor

    Tony Carr

    Insert

    OsTIR1(WT)
    

    Expression

    Yeast

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC119- Padh81-GBP-mCherry(C)-hphMX6-lys1*

    Plasmid

    #89071

    Purpose

    genomic GBP-mCherry epitope C-terminal tagging at lys1+ locus in S. pombe

    Depositor

    Quanwen Jin

    Article

    Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

    Type

    Empty backbone

    Use

    Genomic integration in s. pombe

    Tags

    GBP-mCherry

    Expression

    Yeast

    Available Since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTR-341 pUC19-tNGFR-P2A-PDCD1 N

    Plasmid

    #186074

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    PDCD1 (PDCD1 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-U6-MbCas12a crRNA-BsmBI cassette (BPK4449)

    Plasmid

    #114088

    Purpose

    U6 promoter crRNA entry vector used for all MbCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for MbCas12a crRNAs
    

    Expression

    Mammalian

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC119-Padh81-GBP-mCherry(N)-hphMX6-lys1*

    Plasmid

    #89072

    Purpose

    genomic GBP-mCherry epitope N-terminal tagging at lys1+ locus in S. pombe

    Depositor

    Quanwen Jin

    Article

    Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

    Type

    Empty backbone

    Use

    Genomic integration in s. pombe

    Tags

    GBP-mCherry

    Expression

    Yeast

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUCHR-mClover-smAID-P2A-CD5HA2-bglpA

    Plasmid

    #110655

    Purpose

    Lentiviral vector expressing cell surface HA-tag and monomeric GFP C-terminaly fused with auxin inducible degron

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    mClover-AID-P2A-CD5HA2-bglpA
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. attB-CT-mCherry-linker-attB-AG::pUC-GW-Kan

    Plasmid

    #244900

    Purpose

    Recombinase-mediated integration

    Depositor

    Andrew Bassett

    Article

    Migliori et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    mCherry
    

    Use

    Unspecified

    Available Since

    Nov. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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