We narrowed to 1,842 results for: rigi

1. pS3

   Plasmid

   #50596

   Purpose

   Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5

   Depositor

   Jay Keasling

   Article

   Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

   Inserts

   aroE
   aroD
   aroB
   aroG*
   ppsA
   tktA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available Since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. eNme2C-CBE6c

   Plasmid

   #215831

   Purpose

   Express CBE6c (with eNme2-C variant) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6c-eNme2C-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7-LacO-QF-T7-TetO-TetR_p15A

   Plasmid

   #171661

   Purpose

   Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QF-T7-TetO-TetR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-CBE6a-IVT

   Plasmid

   #215833

   Purpose

   Template for in vitro transcription of CBE6a (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6a-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. hfDECOR-F148A

   Plasmid

   #219546

   Purpose

   Expression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

   Insert

   hfDECOR-F148A
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Changed TadA8e Phenylalanine 148 to Alanine

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. hfDECOR-V106G

   Plasmid

   #219547

   Purpose

   Expression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

   Insert

   hfDECOR-V106G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Changed TadA8e Valine 106 to Glycine

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SaABEmax

   Plasmid

   #119814

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSaCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Zic4-3505:GFP

   Plasmid

   #193001

   Purpose

   Plasmid to generate a transgenic Hydra Zic4 reporter line

   Depositor

   Brigitte Galliot

   Article

   Vogg et al bioRxiv 2021.12.22.47383

   Insert

   Hydra Zic4 promoter
   

   Use

   Hydra transgenesis vector

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-FB-orig BA

   Plasmid

   #13420

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Insert

   orig BA
   

   Use

   Tags

   Gal11P

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAbaMCi-GFP-BsaI_pJMP2748

    Plasmid

    #208888

    Purpose

    Acinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette, sfGFP
    

    Use

    Pir dependent origin of replication

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLys-M1

    Plasmid

    #109382

    Purpose

    Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-VioB-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBT138b-proD

    Plasmid

    #138516

    Purpose

    P3e

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJUMP24-1A(sfGFP)

    Plasmid

    #126971

    Purpose

    Level 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMR-A-6

    Plasmid

    #138520

    Purpose

    P3d

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAC-crRNA-Cm

    Plasmid

    #158711

    Purpose

    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    eforCP (for crRNA cloning)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. eNme2C-CBE6a

    Plasmid

    #215829

    Purpose

    Express CBE6a (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a-eNme2C-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJUMP44-2A(sfGFP)

    Plasmid

    #126988

    Purpose

    Level 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLP3

    Plasmid

    #209958

    Purpose

    Entry vector to store Level 0 broad-host range L. plantarum replication origin parts

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMTU-DO-G418

    Plasmid

    #160007

    Purpose

    Low-copy expression vector for K. marxianus, G418 selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSB1C3-H3

    Plasmid

    #109381

    Purpose

    Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-VioB-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge