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pQE-WT-T3-R5-RRVH

Plasmid

#63623

Purpose

T3-R5-RRVH RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

T3 RRVH RNAP

Mutation

T745K, N748D, L749M, M750I, G753R, H772R, E775V, …

Promoter

T5/Lac

Available Since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pFPV25.1

Plasmid

#20668

Purpose

S. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.

Depositor

Raphael Valdivia

Article

Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Type

Empty backbone

Tags

GFP mut3

Expression

Bacterial

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pPICZalphaB-SapL3

Plasmid

#78171

Purpose

Expression of Saporin L3 in pichia

Depositor

Vern Schramm

Article

Yuan et al Biochemistry. 2015 Jul 28;54(29):4565-74

Insert

Saporin L3

Tags

alpha factor

Expression

Yeast

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pHM129

Plasmid

#225534

Purpose

Encodes destablized mCherry under AR8 promoter, and constitutive BFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

mCherry
BFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG/HA-hBIG1

Plasmid

#226253

Purpose

Expresses N-terminally HA-tagged hBIG1 in mammalian cells

Depositor

Hye-Won Shin

Insert

BIG1 (ARFGEF1 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28CLY1

Plasmid

#21760

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible one SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only

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pwB2b

Plasmid

#32094

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Inserts

BKV VP1
BKV VP2

Expression

Mammalian

Mutation

codon modified

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pHM131

Plasmid

#225530

Purpose

Encodes destablized mCherry under IBRE4 promoter, and constitutive BFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

mCherry
BFP

Expression

Mammalian

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTK222

Plasmid

#13532

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTYT

Tags

See map

Expression

Mammalian

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pBXNH3

Plasmid

#47067

Purpose

FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pYZ183

Plasmid

#98425

Purpose

Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ184

Plasmid

#98426

Purpose

Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSYFP2-SCFP3A

Plasmid

#22905

Depositor

Dorus Gadella

Article

Kremers et al Biochemistry. 2006 May 30. 45(21):6570-8

Insert

SYFP2-SCFP3A

Expression

Mammalian

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

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pET28CLY3

Plasmid

#21762

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 3 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pET28CLY9

Plasmid

#21769

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 9 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pAN1201

Plasmid

#74695

Purpose

Circuit backbone plasmid (LacZa insert)

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Type

Empty backbone

Expression

Bacterial

Available Since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-Neo

Plasmid

#48993

Purpose

Gateway adapted transfer of Neomycin resistance gene

Depositor

Rick Tarleton

Article

Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

Insert

aminoglycoside 3' phosphotransferase

Use

Gateway attb1 & attb2

Available Since

April 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pBB

Plasmid

#53210

Purpose

Contains a 103bp telomeric track

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TTAGGG(n) Track

Promoter

T7

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR221-Hyg

Plasmid

#48994

Purpose

Gateway adapted transfer of Hygromycin resistance gene

Depositor

Rick Tarleton

Article

Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

Insert

hygromycin B phosphotransferase

Use

Gateway attb1 & attb2

Available Since

April 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET28CLY2

Plasmid

#21761

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing two SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Nov. 30, 2009

Availability

Academic Institutions and Nonprofits only