We narrowed to 9,812 results for: Met;
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Plasmid#129049Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA9 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA9 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-10
Plasmid#129050Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA10 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA10 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-11
Plasmid#129051Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA11 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA11 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-12
Plasmid#129052Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA12 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA12 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-2
Plasmid#129042Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA2targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA2 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-3
Plasmid#129043Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA3 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA3 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-4
Plasmid#129044Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA4 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA4 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-5
Plasmid#129045Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA5 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA5 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-6
Plasmid#129046Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA6 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA6 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLgw NKX2-5Y191C-V5-EcoDam
Plasmid#98599PurposeMammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloningDepositorInsertNKX2-5 (Nkx2-5 Mouse)
UseLentiviral; DamidTagsV5 and Dam (DNA adenine methyltransferase)ExpressionMammalianMutationY191C point mutantPromoterHeat Shock Minimal PromoterAvailable SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα-RIIαS97E-IRES-EGFP
Plasmid#183458PurposeReplacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variantDepositorInsertsgRNA and Prkar2a (Prkar2a Rat)
UseLentiviralMutationshRNA-resistant mutations: C135>G, G138>A, …PromotershRNA: H1 / gene: ubiquitinAvailable SinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-CBP(1603-2678)
Plasmid#183774PurposeExpress GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.DepositorAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)
Plasmid#153441PurposeE. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc. XDocB binding mode B knock-out mutant
Tags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialMutationArginine 140 and methionine 144 were mutated to a…PromoterT7 promoterAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-GID1-Cdc42/N12-Cdc42/13C(Q61L)-GAI1-92-mVenus-CAAX
Plasmid#232264PurposeExpress split-Cdc42 fragments that are fused with CIDs and FPsDepositorTagsmCerulean and mVenusExpressionMammalianMutationCdc42-Q61LPromoterCMVAvailable SinceSept. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-GID1-Cdc42/N12-Cdc42/13C(T17N)-GAI1-92-mVenus-CAAX
Plasmid#232265PurposeExpress split-Cdc42 fragments that are fused with CIDs and FPsDepositorTagsmCerulean and mVenusExpressionMammalianMutationCdc42-T17NPromoterCMVAvailable SinceSept. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL(Bad)-pEGFP-C1
Plasmid#166758PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of BadDepositorTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of human Bad …PromoterCMVAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
R989-X30-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D
Plasmid#159557PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G13D (KRAS Human)
TagsHis6-MBP-tevExpressionInsectMutationG13DAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X32-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R
Plasmid#159552PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61RDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationQ61RAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X32-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R
Plasmid#159559PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61RDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R (KRAS Human)
TagsHis6-MBP-tevExpressionInsectMutationQ61RAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X29-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V
Plasmid#159549PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12VDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12V (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12VAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only