We narrowed to 63,814 results for: cell

1. pD649-HAsp-SEMA3F-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156977

   Purpose

   Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA3F (SEMA3F Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75237

   Purpose

   CRISPR/Cas9 plasmid against human NFATc1

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc1 (NFATC1 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Tbg-CES2C-ΔC

   Plasmid

   #200559

   Purpose

   Secreted mouse CES2C driven by heptaocyte-specific promoter in AAV viral vector

   Depositor

   Jonathan Long

   Article

   Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

   Insert

   Mouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)
   

   Use

   AAV

   Tags

   Flag

   Promoter

   TBG promoter

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-sgNotch1#1/Cre

   Plasmid

   #193223

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgNotch1#1 (Notch1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD649-HAsp-IGSF21-COMP5AP-AviTag-9xHis

   Plasmid

   #157429

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   IGSF21 (IGSF21 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. BAP1-010

   Plasmid

   #68366

   Purpose

   stable expression of BAP1 delta 221-238 in mammalian cells

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Parrotta et al J Thorac Oncol. 2017 Apr 4. pii: S1556-0

   Insert

   BAP1 (BAP1 Human)
   

   Expression

   Mammalian

   Mutation

   delta 221-238, splice variant found in human meso…

   Promoter

   CMV

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pD649-HAsp-IL18BP-COMP5AP-AviTag-9xHis

   Plasmid

   #157124

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   IL18BP (IL18BP Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-noSV40-humanEC1.45_p300peak1

   Plasmid

   #173947

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   p300 peak 1 from SOX9 enhancer cluster EC1.45
   

   Use

   Luciferase

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVHC

   Plasmid

   #112614

   Purpose

   promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-Venus (H2BC3 Aequorea victoria, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Promoterless vector, ready for promoter for mamma…

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   No Promoter

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. miniTol2 x EF1a_EKAREN4 + PGK-puro

    Plasmid

    #167824

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLL5.0 ShCavin1-EGFP

    Plasmid

    #187253

    Purpose

    Lentiviral expression of shRNA targeting Cavin1

    Depositor

    Alpha Yap

    Article

    Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

    Insert

    ShRNA for Cavin1 (Cavin1 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    U6

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMIG-hNFATc1/bC - AVITEV

    Plasmid

    #74059

    Purpose

    retroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc1, isoform beta-C (NFATC1 Human)
    

    Use

    Retroviral

    Tags

    AVI-TEV

    Expression

    Mammalian

    Promoter

    pMSCV-LTRs

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD649-HAsp-LAIR2-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156520

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    LAIR2 (LAIR2 Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. TOPO mMecp2-NLucTom

    Plasmid

    #159282

    Purpose

    To tag endogenously and c-terminally mouse Mecp2 with two reporters: NanoLuciferase and TdTomato. It contains 2 homology arms from a castaneus background surrounding the STOP codon.

    Depositor

    Joost Gribnau

    Article

    Mira-Bontenbal et al Stem Cell Reports. 2022 Jan 29. pii: S22

    Insert

    Mecp2 (Mecp2 Mouse)
    

    Tags

    Nluciferase-P2A-TdTomato

    Expression

    Mammalian

    Mutation

    Nluciferase-TdTomato fusion

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-noSV40-mouseEC1.45

    Plasmid

    #173972

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Mouse orthologous SOX9 enhancer cluster EC1.45
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    SV40 promoter

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. NET23 pEGFP-N2 (1174)

    Plasmid

    #62037

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    NET23 (STING1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

17. Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75244

    Purpose

    CRISPR/Cas9 plasmid against human Helios

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Helios (IKZF2 Human)
    

    Use

    CRISPR

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. miniTol2 x EF1a_EKAREN4 + PGK-blast

    Plasmid

    #167825

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4/TO/Strep-HA-ZAKalpha_deltaS

    Plasmid

    #141197

    Purpose

    Expresses Strep-HA-ZAKalpha_deltaS in mammalian cells, can be used to make inducible cell lines

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    deletion of amino acids 670-712

    Promoter

    CMV

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-EHC

    Plasmid

    #112619

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Emerald (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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