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UKNP3.1.1

Plasmid

#98214

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pBad-CA TAG20

Plasmid

#105666

Purpose

expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tag

Depositor

Ryan Mehl

Article

Mehl et al 2020

Insert

human carbonic anhydrase II thermostable variant (CA2 Human)

Use

Tags

6x his tag

Expression

Bacterial

Mutation

TAG stop codon at site 20

Promoter

pBad/arabinose

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA-RagC(S75N)

Plasmid

#99645

Purpose

Bacterial expression of RagA-RagC(S75N)

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

S75N

Promoter

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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C8orf55-CD4d3+4-bio

Plasmid

#73126

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding C8orf55 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

C8orf55 (THEM6 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_C2806

Plasmid

#91091

Purpose

Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Tags

Expression

Mutation

Promoter

FMV 34S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP4.2.1

Plasmid

#98267

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
UKNP2.3.1

Plasmid

#98210

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.21.5

Plasmid

#98203

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pDY-HA1-HA2-neo

Plasmid

#109123

Purpose

FR cassette provider to introduce FR cassette to a BAC by recombineering

Depositor

David Gilbert

Article

Sima et al Nucleic Acids Res. 2018 Feb 28;46(4):181

Inserts

HA1
HA2

Use

Tags

Expression

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)

Plasmid

#122067

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Use

Tags

PA

Expression

Yeast

Mutation

Aspartic acid 195 to alanine; Glutamic acid 196 t…

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG Fzd7 GFP

Plasmid

#66106

Purpose

expression of chicken Fzd7 GFP

Depositor

Martin Scaal, Felicitas Proels

Article

et al Development. 2015 Feb 15;142(4):665-71.

Insert

Fzd7 (FZD7 Chicken)

Use

Chicken expression

Tags

EGFP

Expression

Mutation

Promoter

CAG

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only
pCI-MEG3-del5

Plasmid

#44732

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

Maternally Expressed Gene 3 (MEG3 Human)

Use

Tags

Expression

Mammalian

Mutation

The first 450 nt were deleted from the MEG3 cDNA.

Promoter

hCMV

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-GFP-miR9

Plasmid

#25036

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

mir-9-3 (MIR9-3 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pDN-D2irTN2AG5kwh

Plasmid

#128253

Purpose

Mammalian linearizer gene circuit in a Flp-In expression system based on negative feedback. The hTetR and EGFP is separated by a P2A motif.

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

Beta Globin Intron::hTetR::P2A::EGFP

Use

Synthetic Biology; Flp-in expression vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV D2ir promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense

Plasmid

#46836

Purpose

Express cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pHis-hPim1

Plasmid

#100722

Purpose

Expression of human Pim1 kinase domain in E.coli

Depositor

Byung Il Lee

Article

Lee et al PLoS One. 2013 Jul 31;8(7):e70358. doi:

Insert

Pim1 (PIM1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Residues 29-313 of isoform 2

Promoter

T7 promotor

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pEntry Tshld(FKBP F36V)-GFP

Plasmid

#53260

Purpose

pEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.

Depositor

Matthew Pratt

Article

Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

Insert

Tshld(FKBP F36V)-GFP

Use

Tags

EGFP, Flag, and HA

Expression

Mutation

FKBP F36V

Promoter

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pMMP-puro-Flag-HA-NR6A1

Plasmid

#46833

Depositor

Phil Sharp

Article

Gurtan et al Genes Dev. 2013 Apr 15;27(8):941-54. doi

Insert

NR6A1 (Nr6a1 Mouse)

Use

Retroviral

Tags

Flag-HA, plus 8-amino acid residue linker

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only
SLC9A3R1_PDZ_2

Plasmid

#103933

Purpose

Protein expression and purification of SLC9A3R1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SLC9A3R2 (NHERF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6p-Meis1a

Plasmid

#78144

Purpose

bacterial expression of GST-Meis1a

Depositor

Francesco Blasi

Article

Dardaei et al Proc Natl Acad Sci U S A. 2014 Mar 11;11

Insert

Meis1a (Meis1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only