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pAAV-nEF-Coff/Fon-bREACHes-EYFP

Plasmid

#137147

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Coff/Fon-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBullet-pm-c

Plasmid

#53078

Purpose

destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX FynT-SH2

Plasmid

#46430

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

FynB/FynT (FYN Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 142-251

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Blnk-SH2

Plasmid

#46408

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Blnk (BLNK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 333-456

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 354STOP

Plasmid

#102595

Purpose

Lentivector to express STING V155M delta 354 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M delta 354

Promoter

SFFV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_GPR160

Plasmid

#45274

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

GPR160 (GPR160 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pDB-hCRY1-Luciferase-hvTK-bla

Plasmid

#179444

Purpose

Donor vector to knock in firefly Luciferase C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

Luciferase

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pT2EFCRET(#324)

Plasmid

#184059

Purpose

cyclofen-inducible CRE activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

CRE-ERT2

Use

Zebrafish transient transgenesis

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Xl-EF1a

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_RBMX-eA3A-UdgX-NG-nCas9

Plasmid

#163551

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-SNAP-CD4-bla

Plasmid

#179447

Purpose

Donor vector to knock in SNAPtag C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

SnapTag

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX Grap2-SH2

Plasmid

#46435

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grap2 (GRAP2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 52-159

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pSc1

Plasmid

#80436

Purpose

Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

Depositor

Ervin Welker

Article

Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

Inserts

sp Cas9 gRNA
eGFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV and U6

Available Since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-GOLPH3

Plasmid

#21688

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Gateway entry clone

Mutation

last nucleotide of stop codon removed for c-termi…

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pGEX Abl-SH2 RK

Plasmid

#46403

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Abl RK (Abl1 Mouse)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 139-239

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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Moe-pDONR221

Plasmid

#124239

Purpose

Gateway entry clone of Drosophila Moesin cDNA encoding the D isoform.

Depositor

Peter Vilmos

Article

Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

Insert

Drosophila Moesin (Moe Fly)

Expression

Insect

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Jak1-SH2

Plasmid

#46447

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Jak1 (JAK1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 430-552

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg2(NC)-SH2

Plasmid

#46472

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg2(NC) (PLCG2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 524-749

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pBullet-cg-c

Plasmid

#53070

Purpose

destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pET-pro-siRNA-V2-LMNA

Plasmid

#111088

Purpose

pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human Lamin A/C gene.

Depositor

Linfeng HUANG

Article

Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

Insert

Lamin A/C (LMNA Human)

Expression

Bacterial

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

Plasmid

#184406

Purpose

yeast surface display of the SARS-CoV-2 Beta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

K417N+E484K+N501Y

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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