We narrowed to 12,188 results for: LEA
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Plasmid#96971PurposeS. pombe lem2 knockout cassette with hygro resistance geneDepositorInsertlem2 (lem2 Fission Yeast)
ExpressionBacterialMutationwhole ORF deletion and replaced with hygro resist…PromoterN/AAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMGF172
Plasmid#96998Purposeish1 mCherry insertion cassette with ura4+ geneDepositorInsertish1 (ish1 Fission Yeast)
TagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + ura4(…PromoterN/AAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5C3-T
Plasmid#92022PurposeExpresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting sucA with N-terminal TEV protease cleavage site
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…MutationS219VAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDk4-luciferase fragment B
Plasmid#86659PurposeLuciferase reporter containing CDK4 promoterDepositorPublicationInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationgenerated by cleavage at the KpnI site from 880 (…PromoterCDK7Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(WT)
Plasmid#86576PurposeExpresses cleavable human full-length Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
Tags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130Available sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode81
Plasmid#83758Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode81
UseRetroviralMutationdeletion of T in starting ATG of GFP (please see …Available sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-6NBC-GFP-PGK-Puro-Barcode71
Plasmid#83748Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode71
UseRetroviralMutationdeletion of AA5-233 in GFP (please see depositor …Available sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR-IAA8_no stop
Plasmid#83874PurposeGateway donor vector for IAA8 with no stop codonDepositorInsertindole-3-acetic acid inducible 8 (IAA8 Mustard Weed)
UseGateway CloningMutationno stop codonAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR SS287AA
Plasmid#72703PurposeLentiviral expression of GR Phospho-deficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationSS287AAPromoterubiquitinAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
JW776
Plasmid#64817PurposeBiFC rCUC2DepositorAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIR197
Plasmid#64819PurposeBiFC rCUC2DepositorAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
NET39 Short pmRFP-N2 (451)
Plasmid#61981Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET39 (PPAPDC3 Human)
TagsmRFPExpressionMammalianMutationShort form of NET39PromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
NKP9 pmRFP-N2 (719)
Plasmid#61999Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB689
Plasmid#60133PurposeGreen beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertGrLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB756
Plasmid#60149PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.DepositorInsertYeLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterLEU1prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB752
Plasmid#60155PurposeGreen beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.DepositorInsertGrLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterADE17prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
4MYI
Plasmid#60103PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPVX_084705:MAC04G-C11:C228845
UseBaculovirus expressionTags6x His and TEV cleavage siteMutationSee commentsPromoterpolyhedrinAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEMT-Map7-1783-end
Plasmid#46071DepositorInsertMap7-1783-end (Map7 Mouse)
ExpressionBacterialAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-DKFZP566E144
Plasmid#31593DepositorAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
morc3b_R (OZ576)
Plasmid#35216DepositorInsertZinc finger array targeting morc3b (morc3b Zebrafish)
UseZebrafish targetingAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only