161,600 results

1. pDD162 (Peft-3::Cas9 + Empty sgRNA)

   Plasmid

   #47549

   Purpose

   Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Inserts

   Cas9
   Empty sgRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   U6 and eef-1A.1 (eft-3)

   Available since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDestTol2pA2-U6:gRNA

   Plasmid

   #63157

   Purpose

   Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Type

   Empty backbone

   Use

   CRISPR; Tol2 destination vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT_mEGFP-SNAP-tag2

   Plasmid

   #226513

   Purpose

   Mamalian cell expression of cytosolic mEGFP-SNAP-tag2

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   mEGFP-SNAP-tag2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-LC3-YFP

   Plasmid

   #99571

   Purpose

   Lentiviral expression of YFP-tagged LC3

   Depositor

   Edward Campbell

   Article

   Flavin et al Acta Neuropathol. 2017 May 19. doi: 10.1

   Insert

   LC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX304 CMV HA-HaloTag-FRB-sTurboID (C)

   Plasmid

   #153003

   Purpose

   Expresses low-affinity split-TurboID C-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (C) (G74)
   

   Use

   Lentiviral

   Tags

   FRB, HA, and HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZVsE_SC12

   Plasmid

   #219722

   Purpose

   Zika virus stable E dimer with fusion loop mutation and increased expression

   Depositor

   Brian Kuhlman

   Article

   Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

   Insert

   ZKV sE SC12
   

   Use

   Tags

   8xHis and human serum albumin (HSA)

   Expression

   Mammalian

   Mutation

   G106D, A264W, T267R, S286W, S287P, G29K, A25M

   Promoter

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 35S-sfGFP-nosT

   Plasmid

   #80129

   Purpose

   Transient expression vector for sfGFP in plants

   Depositor

   Yutaka Kodama

   Article

   Fujii et al Plant Biotech. 2015; 32(1): 81-87.

   Type

   Empty backbone

   Use

   Tags

   sfGFP

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Aug. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6a:sgRNA#1

   Plasmid

   #64245

   Purpose

   expresses sgRNA under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. HRE-dUnOHR

   Plasmid

   #201714

   Purpose

   Hypoxia responsive promoter driving UnaG and mOrange

   Depositor

   Friedemann Kiefer

   Article

   Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pttq18

    Plasmid

    #69122

    Purpose

    regulated expression of genes in Escherichia coli

    Depositor

    Michael Stark

    Article

    Stark et al Gene. 1987;51(2-3):255-67.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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