We narrowed to 11,712 results for: NSI

1. HRE-oUnaG

   Plasmid

   #201712

   Purpose

   Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

   Depositor

   Friedemann Kiefer

   Article

   Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

   Insert

   UnaG
   

   Expression

   Mammalian

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF_dCas9

   Plasmid

   #68416

   Purpose

   Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   human EF1[alpha]

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. tTA_BFP

   Plasmid

   #58854

   Purpose

   Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   tTA transcription factor fused to enhanced blue fluorescent protein
   

   Tags

   BFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol-Promoter:NLS-tdMCP-eGFP

   Plasmid

   #86248

   Purpose

   For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

   Depositor

   Florence Marlow

   Article

   Campbell et al Development. 2015 Apr 1;142(7):1368-74.

   Insert

   beta Actin promoter:NLS-tdMCPeGFP
   

   Use

   Used with transposase to make transient or stable…

   Tags

   NLS-tdMCP-eGFP

   Promoter

   Beta Actin

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUA66 PcspA GFP No Linker

   Plasmid

   #105605

   Purpose

   Expresses GFP under temperature sensitive promoter PcspA

   Depositor

   Pamela Silver

   Article

   Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

   Insert

   GFP
   

   Expression

   Bacterial

   Promoter

   PcspA

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. p-dCas9-HDAC1-Hygro

   Plasmid

   #104409

   Purpose

   transient expression of dCas9-HDAC1 fusion protein

   Depositor

   Stephan Beck

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19/N-Vc

   Plasmid

   #183157

   Purpose

   Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAX2

   Plasmid

   #117398

   Purpose

   allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWT35S-mCherry-NLS

   Plasmid

   #194049

   Purpose

   Transient expression of mCherry-NLS in plant cell (Nucleus)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

   Insert

   mCherry
   

   Tags

   SV40 (Nuclear localization signal)

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJKR-O-mphR

    Plasmid

    #62570

    Purpose

    Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. p-dCas9-HDAC1-H141A-Hygro

    Plasmid

    #104417

    Purpose

    transient expression of dCas9-dHDAC1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dHDAC1 (HDAC1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    H141A

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19/Vc-C

    Plasmid

    #183158

    Purpose

    Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas9CyMutL

    Plasmid

    #166981

    Purpose

    Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Inserts

    MutL (mutL )
    CymR
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    pCymRC and pLacI

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pL0M-S-mKOκ-EC18154

    Plasmid

    #137077

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19/N-eCFP

    Plasmid

    #183155

    Purpose

    Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eCFP

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYL322d1/N-Vn

    Plasmid

    #183153

    Purpose

    Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPomyces-FnCpf1

    Plasmid

    #129554

    Purpose

    Contains codon-optimized FnCpf1 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    FnCpf1
    

    Expression

    Bacterial

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL0M-S-mTurquoise2-EC18152

    Plasmid

    #137074

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFH2.214_mCherry-PP7x12

    Plasmid

    #205556

    Purpose

    Express mCherry-PP7x12 (export tag consisting of 12 tandem repeats of PP7 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)/Puro/TagRFP-LL5b

    Plasmid

    #112828

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński, Joshua Sanes

    Article

    Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

    Insert

    LL5b (Phldb2 Mouse)
    

    Tags

    TagRFP

    Expression

    Mammalian

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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