We narrowed to 19,454 results for: cat
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Plasmid#199352PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTagBFP-P2A-ALFA-3CExpressionMammalianMutationWTAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only
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pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP
Plasmid#199362PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-SUMOstar-EGFPExpressionMammalianMutationWTAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-HaloGFP-Ca0.1(Na0.1, 143-144)
Plasmid#198599PurposeGreen fluorescent Ca2+ ion indicator for bacterial expression.DepositorInsertHaloGFP-143-144
ExpressionBacterialPromoteraraBAD promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2082
Plasmid#141913PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFL-EGFP-3xFlag-Fml1
Plasmid#193058PurposeBaculovirus expression of S.pombe Fml1 for recombinant protein productionDepositorAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgTnr#2/Cre
Plasmid#193245PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tnr geneDepositorAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV sgRNA-Hic1 GFP
Plasmid#106953PurposeLentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.DepositorInsertanti-Hic1 sgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgHcn1#2/Cre
Plasmid#193218PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Hcn1 geneDepositorAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
E42_gRunx1_gRNA1_dTet_NGFR
Plasmid#189802PurposeRetroviral delivery of guide RNA against mouse Runx1DepositorInsertgRunx1_gRNA1 (Runx1 Mouse)
UseRetroviralAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIEforRed
Plasmid#185892PurposeIn vivo gene amplification (HapAmp) of EforRed expression module at RPL25 locusDepositorInsertPRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
ExpressionYeastMutationNoneAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMA3735
Plasmid#184848PurposegRNA cloning using blue-white screening for recombinantsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLacZalpha
UseCRISPRExpressionMammalianMutationalpha-fragment of LacZPromoterU6Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-his-Gluc-iLID
Plasmid#172096PurposeExpresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteriaDepositorInsertGaussia Luciferase - iLID
ExpressionBacterialMutationGluc Mutations - M43L, M110LPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_2nd
Plasmid#177230PurposeExpresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinaseDepositorInsertsgNeo1/sgNT1/sgLkb1_2nd
UseLentiviralPromoterbU6/mU6/hU6Available sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_1st
Plasmid#177227PurposeExpresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinaseDepositorInsertsgNeo1/sgNT1/sgLkb1_1st
UseLentiviralPromoterbU6/mU6/hU6Available sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q)-6xHis
Plasmid#176576PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsHexahistidine tag and mVenusExpressionBacterialMutationE184QPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
SUN2delSUN
Plasmid#130835PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
TagsmCherry-FLAG-HAExpressionPlantMutationDeletion of SUN domainPromoterCaMV 35SAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HDR-sphys-3XHA
Plasmid#163690Purposeplasmid for homologous recombination-mediated insertion of triple HA tag into synaptophysin geneDepositorInserttriple HA tag insertion into endogenous mouse synaptophysin gene
UseAAVTagstriple HA tagAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_Crz1(19A)_CLASP_RGS2membrane
Plasmid#133085PurposePlasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.DepositorInsertCrz1*-CLASP
ExpressionBacterial and YeastPromoterpADH1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0380
Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only