We narrowed to 26,401 results for: Vit

1. TBK1 gRNA (BRDN0001145663)

   Plasmid

   #76361

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TBK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TBK1 (TBK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

   Plasmid

   #63139

   Purpose

   Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

   Depositor

   Titia Sixma

   Article

   Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

   Inserts

   Ring1b (Ring1 Mouse)
   Bmi1 (Bmi1 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Residues 1-109 and Residues 1-159

   Promoter

   tac

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

3. RPS6KA2 gRNA (BRDN0001147334)

   Plasmid

   #75732

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RPS6KA2 (RPS6KA2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

   Plasmid

   #115787

   Purpose

   Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   FZD4 (FZD4 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-Sec22b-shR

   Plasmid

   #208358

   Purpose

   Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b

   Depositor

   Paula Nunes-Hasler

   Article

   Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

   Insert

   Sec22b (Sec22b Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   four silent mutations introduced to render shRNA …

   Promoter

   CMV

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-MBP-TEV-PWWP_DNMT3A

   Plasmid

   #186970

   Purpose

   Bacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.

   Depositor

   Brian Liau

   Article

   Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

   Insert

   DNMT3A PWWP domain (DNMT3A Synthetic, Human)
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   PWWP domain (residues 278–427)

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1 shETV4-A

   Plasmid

   #74975

   Purpose

   shETV4 shRNA

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   ETV4 (ETV4 Human)
   

   Use

   RNAi

   Expression

   Mammalian

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.5 shETV4-B

   Plasmid

   #74976

   Purpose

   shETV4 shRNA

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   ETV4 (ETV4 Human)
   

   Use

   RNAi

   Expression

   Mammalian

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-DIO-P-Ef1a-mCherry-T2A-RPL22-1xV5

   Plasmid

   #170328

   Purpose

   Double-floxed inverse Orientation V5 and mCherry tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. eGFP Depdc5 pLJM1

    Plasmid

    #46380

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Differs from Depdc5 isoform 1 by 10 amino acids …

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

11. PKN1 gRNA (BRDN0001162558)

    Plasmid

    #76278

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN1 (PKN1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. ACVR1-TAAS-R206H

    Plasmid

    #206184

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S192A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    S190A, S192A, R206H

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. YFP-Parkin C431N

    Plasmid

    #46924

    Purpose

    expresses mutant Parkin with YFP tag in mammalian cells

    Depositor

    Richard Youle

    Article

    Lazarou et al J Cell Biol. 2013 Jan 21;200(2):163-72.

    Insert

    Park2 (PRKN Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed cysteine 431 to asparagine

    Promoter

    CMV

    Available Since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. GPR141-DuET

    Plasmid

    #213263

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR141 (GPR141 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSV-CaMKIV(1-313)

    Plasmid

    #45063

    Purpose

    Expression of constit active CaMKIV

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    Cam Kinase IV(1-313) (Camk4 Mouse)
    

    Expression

    Mammalian

    Mutation

    truncated at amino acid 313*

    Promoter

    RSV

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-CoV2-Omicron BA.1 spike

    Plasmid

    #194602

    Purpose

    Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

    Depositor

    Ben Murrell

    Article

    Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

    Insert

    CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GPR20-DuET

    Plasmid

    #213275

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR20 (GPR20 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PGK1 gRNA (BRDN0001144730)

    Plasmid

    #77980

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PGK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PGK1 (PGK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+ Zf ctnna1L344P

    Plasmid

    #185407

    Purpose

    Zebrafish ctnna1 bearing a point mutation in the vinculin binding site. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    ctnna1 (ctnna1 Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Expression

    Mammalian

    Mutation

    changed Leucine 344 to Proline

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. hA3A-BE3 (pRZ215)

    Plasmid

    #131314

    Purpose

    CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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