Addgene

We narrowed to 355 results for: NS1

Showing: 121 - 150 of 355 results

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pC0.174

Plasmid

#119641

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS1 Down Flank

Use

Synthetic Biology

Tags

Expression

Mutation

bp 658 G to A

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAM5081

Plasmid

#85107

Purpose

Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

YFP-KaiC

Use

Tags

YFP

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAM4951 (pCV0063)

Plasmid

#120084

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-puro SHP2 WT

Plasmid

#8329

Depositor

Ben Neel

Article

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-SHP2WT

Plasmid

#12283

Depositor

Anton Bennett

Article

Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

Insert

SHP-2WT (PTPN11 Human)

Use

Tags

Myc (not canonical: KLISEEDL)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pBABE-puro SHP2 E76A

Plasmid

#8331

Depositor

Ben Neel

Article

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

E76A (activated mutant)

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pBABE-puro SHP2 D61A

Plasmid

#8330

Depositor

Ben Neel

Article

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

D61A (activated mutant)

Promoter

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
pSicoR-PTPN11i1

Plasmid

#59612

Purpose

Expression of shRNA against human PTPN11

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPN11 (PTPN11 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-PTPN11i2

Plasmid

#59613

Purpose

Expression of shRNA against human PTPN11

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPN11 (PTPN11 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pENTR2b-PTPN11

Plasmid

#215476

Purpose

Gateway entry vector with Shp2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

none

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-PTPN11-Clover

Plasmid

#215519

Purpose

Gateway entry vector with clover-tagged Shp2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Gateway entry vector

Tags

Clover

Expression

Mutation

Promoter

none

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-PTPN11

Plasmid

#215523

Purpose

Expression vector with Shp2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-PTPN11-Clover

Plasmid

#215525

Purpose

Expression vector with clover-tagged Shp2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Tags

Clover

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pIRES2-EGFP-SHP2CS

Plasmid

#12284

Depositor

Anton Bennett

Article

Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

Insert

SHP-2CS (PTPN11 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Cysteine 459 mutated to Serine

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-SHP2DA

Plasmid

#12286

Depositor

Anton Bennett

Article

Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

Insert

SHP-2DA (PTPN11 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Aspartic Acid 425 mutated to Alanine

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pHR-SV40-mCherry-SHP1 (SHP2-cSH2)

Plasmid

#180822

Purpose

Expression of N-terminal mCherry tagged human SHP1 with its cSH2 replaced by SHP2-cSH2

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

SHP1 (SHP2-cSH2) (PTPN6, PTPN11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SV40

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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MDH1-shSHP2-3-H2Kb2

Plasmid

#17854

Depositor

Chang-Zheng Chen

Article

Li et al Cell. 2007 Apr 6. 129(1):147-61.

Insert

shSHP2 (PTPN11 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM4731

Plasmid

#85127

Purpose

NSI-NT expressing KaiB from native promoter. Tested by Topcount: Displays WT rhythms.

Depositor

Susan Golden

Article

Chang et al Science. 2015 Jul 17;349(6245):324-8. do

Insert

PkaiBC-KaiB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR2b-PTPN11(D425A,C459S)

Plasmid

#215515

Purpose

Gateway entry vector with a phosphatase-inactive Shp2 mutant (D425A,C459S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D425A, C459S (Phosphatase inactive)

Promoter

none

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac (GST-SNAP-Shp2)

Plasmid

#177897

Purpose

Baculo expression of GST tag fused to SNAP tag and human Shp2

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 (PTPN11 Human)

Use

Tags

TEV-GST-PreScission cut site-SNAP tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac(His10-Shp2)

Plasmid

#177899

Purpose

Baculo expression of His10 tag fused to human Shp2

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 (PTPN11 Human)

Use

Tags

10xHis-TEV-SNAP tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SV40-mCherry-SHP1 (SHP2-nSH2, cSH2)

Plasmid

#180823

Purpose

Expression of N-terminal mCherry tagged human SHP1 with its nSH2 and cSH2 replaced by nSH2 and cSH2 of SHP2

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

SHP1 (SHP2-nSH2, cSH2) (PTPN6, PTPN11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SV40

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P2(GST-SNAP-Shp2tSH2)

Plasmid

#177907

Purpose

Baculo expression of GST tag fused to SNAPtag and human Shp2 tandem SH2 domains

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 tandem SH2 domains (aa 6-218 only) (PTPN11 Human)

Use

Tags

GST-PreScission cut site-SNAP tag

Expression

Bacterial

Mutation

Promoter

tac

Available since

Availability

Academic Institutions and Nonprofits only

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pGEX tensin-SH2

Plasmid

#46525

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

tensin (TNS1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1451-1588

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHP-2(N)-SH2

Plasmid

#46498

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(N) (PTPN11 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-104

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SHP-2(NC)-SH2

Plasmid

#46499

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(NC) (PTPN11 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-222

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SHP-2(C)-SH2

Plasmid

#46497

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(C) (PTPN11 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 101-222

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
XX01CAPN1A-c001

Plasmid

#175491

Purpose

Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.

Depositor

Opher Gileadi

Insert

CAPN1 / CAPNS1 (CAPN1 Human)

Use

Tags

AviTag and His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
XX01CAPN2A-c001

Plasmid

#175492

Purpose

Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.

Depositor

Opher Gileadi

Insert

CAPN2 / CAPNS1 (CAPN2 Human)

Use

Tags

AviTag and His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
piHGpd/p82

Plasmid

#1105

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

wild-type HSP82 regulated by the strong constitut…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only