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We narrowed to 5,049 results for: golden gate

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Showing: 201 - 250 of 5049 results

  1. pOGG010

    Plasmid
    #113984
    Purpose
    Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    RK2 (IncP)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113984-plasmid-map-sequence-id-223395

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  2. pOGG011

    Plasmid
    #113985
    Purpose
    Vector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectors
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    pBBR1
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113985-plasmid-map-sequence-id-223389

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  4. pICSL13003

    Plasmid
    #245653
    Purpose
    Level 0 Golden Gate part 6GAL4UAS (Dex inducible expression system) promoter, GGAG - CCAT
    Depositor
    Mark Youles
    Insert
    6GAL4UAS (Dex inducible expression system) promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    245653-plasmid-map-sequence-id-495394

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  5. pICSL30070

    Plasmid
    #245572
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag V5-TurboID Fusion N-TAG (includes a 3' 3xGGGS Linker)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    245572-plasmid-map-sequence-id-501811

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  6. pICSL30043

    Plasmid
    #245548
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Sv40 Nuclear Localization Signal (MASSPPKKKRKVSWKM)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    245548-plasmid-map-sequence-id-498365

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  7. pICSL30002

    Plasmid
    #245530
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag SplitYFP tag -C terminus of YFP
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    245530-plasmid-map-sequence-id-495355

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  8. pICSL12002

    Plasmid
    #245626
    Purpose
    Level 0 Golden Gate part pAt6: Ubiquitin / AtUbl5 (At5g42300) promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    pAt6: Ubiquitin / AtUbl5 (At5g42300) promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    245626-plasmid-map-sequence-id-495368

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  9. pICSL12030

    Plasmid
    #245637
    Purpose
    Level 0 Golden Gate part pAt1: Tip Delta (AT3g16240) promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    pAt1: Tip Delta (AT3g16240) promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Jan. 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    245637-plasmid-map-sequence-id-495381

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  10. pICSL61004

    Plasmid
    #245681
    Purpose
    Level 0 Golden Gate part 3' Extensin-Truncated-EU (TerP1) terminator P1, GCTT - TAGA
    Depositor
    Mark Youles
    Insert
    3' Extensin-Truncated-EU (TerP1) terminator P1
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245681-plasmid-map-sequence-id-495768

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  11. pICSL62002

    Plasmid
    #245683
    Purpose
    Level 0 Golden Gate part3' Extensin-Full length (TerP2) terminator P2, TAGA - CGCT
    Depositor
    Mark Youles
    Insert
    3' Extensin-Full length (TerP2) terminator P2
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245683-plasmid-map-sequence-id-495763

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  12. pICSL62003

    Plasmid
    #245684
    Purpose
    Level 0 Golden Gate part 3' Extensin-Truncated (TerP2) terminator P2, TAGA - CGCT
    Depositor
    Mark Youles
    Insert
    3' Extensin-Truncated (TerP2) terminator P2
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245684-plasmid-map-sequence-id-495760

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  13. pICSL30069

    Plasmid
    #245571
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Twin Streptavidin-TurboID Fusion N-TAG (includes a 3' 3xGGGS Linker)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245571-plasmid-map-sequence-id-495565

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  14. pICSL30071

    Plasmid
    #245573
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag 6HIS-AviTAG-MBP-C3 (Maltose Binding Protein)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245573-plasmid-map-sequence-id-495567

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  15. pICSL30059

    Plasmid
    #245562
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245562-plasmid-map-sequence-id-495558

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  16. pICSL30060

    Plasmid
    #245563
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving GB1 tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245563-plasmid-map-sequence-id-495559

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  17. pICSL30061

    Plasmid
    #245564
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving MBP tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245564-plasmid-map-sequence-id-495560

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  18. pICSL12048

    Plasmid
    #245645
    Purpose
    Level 0 Golden Gate part Single-34S FMV (Figwort Mosaic Virus). promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    Single-34S FMV (Figwort Mosaic Virus). promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245645-plasmid-map-sequence-id-495541

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  19. pICSL12028

    Plasmid
    #245635
    Purpose
    Level 0 Golden Gate part pAt2: Rib Prot16 (At4g34620) promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    pAt2: Rib Prot16 (At4g34620) promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245635-plasmid-map-sequence-id-495533

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  20. pICSL50058

    Plasmid
    #245613
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag TurboID+ V5. With GUS Intron and GGGS(x3) linker
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245613-plasmid-map-sequence-id-495523

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  21. pICSL50047

    Plasmid
    #245603
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag N-terminal portion of split Luciferase
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245603-plasmid-map-sequence-id-495518

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  22. pICSL50048

    Plasmid
    #245604
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag C-terminal portion of split Luciferase
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245604-plasmid-map-sequence-id-495519

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  23. pICSL50034

    Plasmid
    #245592
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag eGFP
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation, also c…
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245592-plasmid-map-sequence-id-495512

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  24. pICSL50005

    Plasmid
    #245578
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag YFP tag (yellow variant of GFP)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245578-plasmid-map-sequence-id-495501

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  25. pICSL60025

    Plasmid
    #245673
    Purpose
    Level 0 Golden Gate part Rb7MAR (D144-437 variant) terminator, GCTT - CGCT
    Depositor
    Mark Youles
    Insert
    Rb7MAR (D144-437 variant) terminator
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245673-plasmid-map-sequence-id-495495

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  26. pICSL30063

    Plasmid
    #245566
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving HF tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245566-plasmid-map-sequence-id-495490

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  27. pICSL30064

    Plasmid
    #245567
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving STREPII tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245567-plasmid-map-sequence-id-495491

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  28. pICSL30065

    Plasmid
    #245568
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving SPY tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245568-plasmid-map-sequence-id-495492

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  29. pICSL30062

    Plasmid
    #245565
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving SUMO tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245565-plasmid-map-sequence-id-495489

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  30. pICSL13020

    Plasmid
    #245664
    Purpose
    Level 0 Golden Gate part EC1.2-EN-EC1.1 Enhancer Fusion promoter, GGAG - CCAT
    Depositor
    Mark Youles
    Insert
    EC1.2-EN-EC1.1 Enhancer Fusion promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245664-plasmid-map-sequence-id-495486

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  31. pICSL12080

    Plasmid
    #245650
    Purpose
    Level 0 Golden Gate part EC1.2-EN-EC1.1 Enhancer Fusion promoter, GGAG - AATG
    Depositor
    Tom Lawrenson, Mark Youles
    Insert
    EC1.2-EN-EC1.1 Enhancer Fusion promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245650-plasmid-map-sequence-id-495475

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  32. pICSL30001

    Plasmid
    #245529
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag SplitYFP tag- N terminus of YFP
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245529-plasmid-map-sequence-id-495461

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  33. pICSL12007

    Plasmid
    #245628
    Purpose
    Level 0 Golden Gate part pAt3: Cysteine Synthase (At3g61440) promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    pAt3: Cysteine Synthase (At3g61440) promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245628-plasmid-map-sequence-id-495458

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  34. pOPARA2

    Plasmid
    #200417
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200417-plasmid-map-sequence-id-394144

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  35. pOPARA1

    Plasmid
    #200416
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200416-plasmid-map-sequence-id-394143

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  36. pTALE64

    Plasmid
    #35189
    Purpose
    Golden Gate Compatible TALE Transcription Factor using VP64 Domain
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Transcription Factor
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, VP64 Domain
    Promoter
    CMV
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    35189-plasmid-map-sequence-id-68899

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  37. pICSL41258_CP

    Plasmid
    #245691
    Purpose
    Level 0 Golden Gate Signal Peptide acceptor with AmilCP negative selection cassette AATG - AGGT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245691-plasmid-map-sequence-id-495769

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  38. pICSL61003

    Plasmid
    #245680
    Purpose
    Level 0 Golden Gate part 3' Extensin-Full length-IEU (TerP1) terminator P1, GCTT - TAGA
    Depositor
    Mark Youles
    Insert
    3' Extensin-Full length-IEU (TerP1) terminator P1
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245680-plasmid-map-sequence-id-495766

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  39. pICSL12049

    Plasmid
    #245646
    Purpose
    Level 0 Golden Gate part Double (enhancer)-34S FMV (Figwort Mosaic Virus). promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    Double (enhancer)-34S FMV (Figwort Mosaic Virus). promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245646-plasmid-map-sequence-id-495542

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  40. pICSL41276_CP

    Plasmid
    #245693
    Purpose
    Level 0 Golden Gate Terminator acceptor with AmilCP negative selection cassette GCTT - CGCT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245693-plasmid-map-sequence-id-495522

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  41. pICSL41295_CP

    Plasmid
    #245694
    Purpose
    Level 0 Golden Gate Promoter acceptor with AmilCP negative selection cassette GGAG - AATG
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245694-plasmid-map-sequence-id-495515

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  42. pICSL50017

    Plasmid
    #245579
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag YFP with nuclear localisation signal from Simian virus large T-antigen
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245579-plasmid-map-sequence-id-495502

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  43. pICSL60027

    Plasmid
    #245675
    Purpose
    Level 0 Golden Gate part Extensin-Truncated-EU + NbACT + Rb7MAR (D144-437 variant) terminator, GCTT - CGCT
    Depositor
    Mark Youles
    Insert
    Extensin-Truncated-EU + NbACT + Rb7MAR (D144-437 variant) terminator
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245675-plasmid-map-sequence-id-495497

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  44. pCVL RipTAL(Nd154C63) SFFV Xba-Sal linker

    Plasmid
    #50627
    Purpose
    pRipTAL plasmid compatible with Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50627-plasmid-map-sequence-id-79594

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  49. pTALE64G

    Plasmid
    #35188
    Purpose
    Golden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP addition
    Depositor
    Gang Bao
    Insert
    TAL Effector-VP64-T2A-eGFP
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, VP64 Domain, T2A-GFP
    Promoter
    CMV
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    35188-plasmid-map-sequence-id-42839

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  50. pICSL41264_CP

    Plasmid
    #245692
    Purpose
    Level 0 Golden Gate Signal Peptide + Coding Sequence acceptor with AmilCP negative selection cassette AGGT - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245692-plasmid-map-sequence-id-495765

    Enlarge

Showing: 201 - 250 of 5049 results