Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,038 results for: B ALPHA

Showing: 81 - 100 of 1038 results

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pAAV-anti-TNFalpha(Ozoralizumab) PAGER(Gq)

Plasmid

#230015

Purpose

Expresses anti-TNFalpha PAGER(Gq) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-Ozoralizumab-TEVcs-ALFA-hM1D

Use

AAV

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-TNFalpha(Ozoralizumab) PAGER(FL)

Plasmid

#230024

Purpose

Expresses anti-TNFalpha PAGER(FL) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

pAAV-MT3-Ozoralizumab-TEVcs-ALFA-hM4DxcpGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti6-AMPK alpha1 (1-312)

Plasmid

#162131

Purpose

Expression vector for constitutively active AMPK alpha 1

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2016 Apr;18(4):431-42. do

Insert

AMPK alpha1 (1-312) (PRKAA1 Rat)

Use

Lentiviral

Tags

V5

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha1 clone1

Plasmid

#162119

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha1 clone2

Plasmid

#162120

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha1 clone3

Plasmid

#162121

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

Plasmid

#128749

Purpose

Expresses control gRNA

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

Plasmid

#128755

Purpose

Expresses CYRANO gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

CYRANO sgRNA (OIP5-AS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha2 clone1

Plasmid

#162122

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha2 clone2

Plasmid

#162123

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha2 clone3

Plasmid

#162124

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPICZalphaB-SapL3

Plasmid

#78171

Purpose

Expression of Saporin L3 in pichia

Depositor

Vern Schramm

Article

Yuan et al Biochemistry. 2015 Jul 28;54(29):4565-74

Insert

Saporin L3

Tags

alpha factor

Expression

Yeast

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-TNFalpha(Ozoralizumab) PAGER(TF)

Plasmid

#230001

Purpose

Expresses anti-TNFalpha PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

IL2SP-Aro6-Ozoralizumab-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4

Use

AAV

Expression

Mammalian

Mutation

V360A/R361A on KORD

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Rat Alpha2 delta-1 mutMIDAS pMT2

Plasmid

#58730

Purpose

expression of rat alpha2 delta-1 with MIDAS site mutations in mammalian cells

Depositor

Annette Dolphin

Article

Hoppa et al Nature. 2012 May 13;486(7401):122-5. doi

Insert

alpha2 delta-1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

MIDAS site mutations (D259VSGS to AVAGA)

Promoter

SV40

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

Plasmid

#128760

Purpose

Expresses PARN gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PARN sgRNA #2 (PARN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

Plasmid

#128758

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #3 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

Plasmid

#128756

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

Plasmid

#128757

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

Plasmid

#71396

Purpose

2Gi2R assay

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Inserts

2xGFP:NES
2xRFP:NLS

Use

Lentiviral

Tags

IRES

Expression

Mammalian

Promoter

EF1alpha and IRES

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)

Plasmid

#160626

Purpose

TU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domains

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35s-dCas9:TV-Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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Showing: 81 - 100 of 1038 results

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