We narrowed to 147 results for: EcoRI

1. pLpC-L-IFP-F[2] (mammalian)

   Plasmid

   #52902

   Purpose

   Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   L-IFP-F[2]
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Unknown

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pMD19T-psba1-Ppsba2-dCas9-SpR

   Plasmid

   #73220

   Purpose

   Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

   Depositor

   Paul Hudson

   Article

   Yao et al ACS Synth Biol. 2015 Dec 28.

   Insert

   dCas9 from S. pyogenes
   

   Use

   Tags

   c-myc

   Expression

   Bacterial

   Mutation

   Silent mutation at bp 1341 A->C to remove an E…

   Promoter

   PpsbA2

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJM601-TRA-2::FLAG

   Plasmid

   #27129

   Depositor

   Ding Xue

   Article

   Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

   Insert

   TRA-2 FLAG (tra-2 Nematode)
   

   Use

   Tags

   3x FLAG

   Expression

   Worm

   Mutation

   Replaced TRA-2 stop codon with KpnI and EcoRI and…

   Promoter

   Available since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. Mix.2-lux M-p53BE

   Plasmid

   #20894

   Depositor

   Stefano Piccolo

   Article

   Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

   Insert

   -290 Mix.2 M-p53BE promoter luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   point mutation of the p53 binding element (p53BE)…

   Promoter

   Available since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

5. mc2 blast hPGK

   Plasmid

   #206229

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mc2 hygro hPGK

   Plasmid

   #206228

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. mc2 puro hPGK

   Plasmid

   #206227

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mc2 neo hPGK

   Plasmid

   #206226

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mc2 hygro EF1a

   Plasmid

   #206222

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mc2 hygro

    Plasmid

    #206216

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C2-tepsin

    Plasmid

    #196707

    Purpose

    Expresses GFP-tepsin in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

    Insert

    tepsin (TEPSIN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent substitution in codon 199 to eliminate int…

    Promoter

    CMV

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-STAT3-linker-TagGFP2

    Plasmid

    #121427

    Purpose

    WT STAT3 inserted using Infusion Cloning into MluI and EcoRI digested pLenti-Origene-Nrf21.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    STAT3-linker-TagGFP2
    

    Use

    Tags

    TagGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBT9-tepsin

    Plasmid

    #196647

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-tepsin fusion protein

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

    Insert

    tepsin (TEPSIN Human)
    

    Use

    Yeast two-hybrid vector

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    silent substitution in codon 199 to eliminate int…

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. KBB215

    Plasmid

    #185068

    Purpose

    SST::WAK2 EcoRI/ScaI fragment (secretion confirmed)

    Depositor

    Ken Belanger

    Article

    Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

    Insert

    WAK2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. YF-Cdc42(CA)

    Plasmid

    #20152

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Cdc42(CA) (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cdc42H was inserted into the multiple cloning sit…

    Promoter

    Available since

    March 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-MAGE-2-429

    Plasmid

    #89129

    Purpose

    bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

    Depositor

    Elizabeth Wilson

    Article

    Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

    Insert

    MAGE-A11 (MAGEA11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pLpC-Reg-L-IFP-F[2]+Venus (mammalian)

    Plasmid

    #52903

    Purpose

    Binary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Reg-L-IFP-F[2]+Venus
    

    Use

    Retroviral

    Tags

    L-IFP-F[2]

    Expression

    Mammalian

    Mutation

    Promoter

    Unknown

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pPV230.13

    Plasmid

    #14902

    Depositor

    James Lok

    Article

    Li et al Int J Parasitol. 2006 May 31. 36(6):671-

    Insert

    Strongyloides stercoralis transitional endoplasmic reticulum ATPase promoter region
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Ss era-1 promoter region as a HindIII-PstI fragme…

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)

    Plasmid

    #40341

    Depositor

    Alexander Dent

    Article

    Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

    Insert

    Blimp1 promoter (mutated) (PRDM1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Two AP-1 sites at -1813 and -1647 replaced with B…

    Promoter

    Blimp1

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1 Flag-TUBG2

    Plasmid

    #229855

    Purpose

    TUBG2

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

    Insert

    TUBG2 (TUBG2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Removal of the internal TUBG2-EcoRI site by chang…

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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