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pEM705 Gst (Ser)

Plasmid

#109354

Purpose

GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gst chimera

Use

Tags

None

Expression

Mammalian

Mutation

Changed Cysteine at position 391 to Serine (Pertu…

Promoter

CAG

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2 HRD

Plasmid

#207544

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human WIPI2 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oDi

Plasmid

#124661

Purpose

Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oDi

Use

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oTet

Plasmid

#124663

Purpose

Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oTet

Use

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyCatcher002-oTri

Plasmid

#124662

Purpose

Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oTri

Use

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Rod Opsin

Plasmid

#109361

Purpose

Human rod opsin

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin (RHO Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oHex

Plasmid

#124670

Purpose

Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oHex

Use

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyCatcher002-oPent

Plasmid

#124664

Purpose

Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oPent

Use

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM705 Gso (Ser)

Plasmid

#109353

Purpose

GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gso chimera

Use

Tags

None

Expression

Mammalian

Mutation

Changed Cysteine at position 391 to Serine (Pertu…

Promoter

CAG

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM705 Gsi (Ser)

Plasmid

#109352

Purpose

GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsi chimera

Use

Tags

None

Expression

Mammalian

Mutation

Changed Cys at position 391 to Serine (Pertussis …

Promoter

CAG

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyCatcher002-oHept

Plasmid

#124671

Purpose

Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oHept

Use

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM705 Gso (Cys)

Plasmid

#109375

Purpose

GsX chimera with Gαo C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gso chimera

Use

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEM705 Gsi (Cys)

Plasmid

#109373

Purpose

GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsi chimera

Use

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-ATG9A HRD

Plasmid

#203222

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG9 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEM705 Gst (Cys)

Plasmid

#109376

Purpose

GsX chimera with Gαt C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gst chimera

Use

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcUW51-gE2

Plasmid

#11621

Depositor

Pamela Bjorkman

Article

Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

Insert

HSV-1 glycoprotein E

Use

Tags

His

Expression

Insect

Mutation

deleted amino acids 391-550

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
Halo-ATG16L1 HRD

Plasmid

#207537

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1 EGFP-p62 HRD

Plasmid

#207550

Purpose

Homologous recombination donor to integrate and expression cassette for eGFP-p62 in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-P62

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSuper TR G414C

Plasmid

#207612

Purpose

U3 promoter driven expression of the telomerase RNA with a G414C CAB box mutation

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

Telomerase RNA G414C

Use

Tags

Expression

Mammalian

Mutation

Promoter

U1

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-ATG2A HRD

Plasmid

#207540

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG2A locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG2A locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge