We narrowed to 172 results for: LPP
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Plasmid#235098PurposeQuantitative bacterial two-hybrid (qB2H). Expresses IP1.DepositorInsertIP1
UseSynthetic Biology; Quantitative bacterial two-hyb…TagsrpoAExpressionBacterialPromoterlpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN516 pBR-rpoA-IP2
Plasmid#235099PurposeQuantitative bacterial two-hybrid (qB2H). Expresses IP2.DepositorInsertIP2
UseSynthetic Biology; Quantitative bacterial two-hyb…TagsrpoAExpressionBacterialPromoterlpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN517 pBR-rpoA-IP3
Plasmid#235100PurposeQuantitative bacterial two-hybrid (qB2H). Expresses IP3.DepositorInsertIP3
UseSynthetic Biology; Quantitative bacterial two-hyb…TagsrpoAExpressionBacterialPromoterlpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN518 pBR-rpoA-IP4
Plasmid#235101PurposeQuantitative bacterial two-hybrid (qB2H). Expresses IP4.DepositorInsertIP4
UseSynthetic Biology; Quantitative bacterial two-hyb…TagsrpoAExpressionBacterialPromoterlpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKB237.1
Plasmid#170031PurposeEncodes LacI-controlled expression of surface-displayed CCL19 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CCL19 Δ alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB235.4
Plasmid#170026PurposeEncodes LacI-controlled expression of surface-displayed CCL20 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CCL20 Δ alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailabilityAcademic Institutions and Nonprofits only -
pKB236.1
Plasmid#170028PurposeEncodes LacI-controlled expression of surface-displayed TCP fragment lacking C-terminal alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
TCP Δ C-terminal alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailabilityAcademic Institutions and Nonprofits only -
pKB236.2
Plasmid#170029PurposeEncodes LacI-controlled expression of surface-displayed TCP C-terminal alpha-helix fragment and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
TCP C-terminal alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailabilityAcademic Institutions and Nonprofits only -
pKB237
Plasmid#170030PurposeEncodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDULE-ABK
Plasmid#49086PurposeA pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experimentsDepositorInsertstRNA synthetase
tRNA for a photocrosslinking amino acid
ExpressionBacterialMutationL274M, C313A, Y349FPromoterGlnRS and lppAvailable SinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKB238
Plasmid#170033PurposeEncodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBRα-σ70 D581G
Plasmid#53732PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertRNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
ExpressionBacterialMutationThe sigma 70 moiety carries the D581G substitutionPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKB235
Plasmid#170024PurposeEncodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDule-tfmF A65V S158A
Plasmid#85484PurposeMachinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNADepositorInserttri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
TagsNoneExpressionBacterialMutationY32V F108W Q109M I159M with 65Val and 158AlaPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule2-pCNF
Plasmid#85495PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
TagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule-pCNF
Plasmid#85494PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
TagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule2-para-aminoPhe
Plasmid#85503PurposePlasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertp-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
TagsNoneExpressionBacterialMutationY32T, E107T, D158P, I159L, L162APromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule-para-aminoPhe
Plasmid#85502PurposePlasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertp-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
TagsNoneExpressionBacterialMutationY32T, E107T, D158P, I159L, L162APromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
VN1852 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP4_Ddcm_WeaKRBS_TIR51_KanR
Plasmid#235118PurposeQuantitative bacterial two-hybrid (qB2H). Expresses ASf1B and IP4.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterLtetO and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN1316 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP4_Ddcm_eGFP-wcKanR
Plasmid#235113PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP4.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterLtetO and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only