We narrowed to 119 results for: MAF
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Plasmid#17559DepositorInsertFoxo1 6KQ (Foxo1 Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
AY27_pU6.gRNA.CLYBL
Plasmid#199238PurposeExpression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. pyogenes gRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNAAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo1-WT 6KR
Plasmid#17560DepositorInsertFoxo1 6KR (Foxo1 Mouse)
UseTagsFlag and MycExpressionMammalianMutationK242, K245, K259, K262, K271 and K291 were replac…PromoterCMVAvailable sinceOct. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo1ADA(pCMV5)
Plasmid#12149DepositorInsertFoxo1 ADA (Foxo1 Mouse)
UseTagsFlag and MycExpressionMammalianMutationThreonine 24 to Alanine (A) and Serine 253 to Asp…PromoterCMVAvailable sinceJune 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
BB44_pmc.DonorR5.TS
Plasmid#199223PurposeDonor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locusDepositorInsertExpression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo1-WT 6KQ
Plasmid#17562DepositorInsertFoxo1 6KQ (Foxo1 Mouse)
UseTagsFlag and MycExpressionMammalianMutationK242, K245, K259, K262, K271 and K291 were replac…PromoterCMVAvailable sinceOct. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
AM77_pU6.Sa-gRNA.CLYBL
Plasmid#199237PurposeExpression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. aureus gRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNAAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AY22_pgRNA.R5.1
Plasmid#100294PurposeCCR5-targeting gRNA expression plasmidDepositorInsertgRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6 RNA Pol III promoter human snRNAAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
AV44_pCAG.Cas9-D10A.gRNA.S1
Plasmid#199258PurposeExpression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNADepositorInsertsSpCas9-D10A nickase
AAVS1-targeting gRNA
UseCRISPRTagsSV40 NLSExpressionMammalianMutationD10APromoterCAG promoter and RNA polymerase III promoter for …Available sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AV15_pCAG.Cas9.gRNA.S1
Plasmid#199259PurposeExpression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNADepositorInsertsSpCas9 nuclease
AAVS1-targeting gRNA
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterCAG promoter and RNA polymerase III promoter for …Available sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sema5a-AP-His
Plasmid#72035PurposeExpresses the extracellular region of the Sema5A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema5a (Sema5a Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Myc-Foxo1-WT 6KR
Plasmid#17558DepositorInsertFoxo1 6KR (Foxo1 Mouse)
UseTagsMycExpressionMammalianMutationK242R, K246R, K259R, K262R, K271R, K291RPromoterCMVAvailable sinceOct. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
AL08_pgRNA.DMD
Plasmid#100293PurposeDMD intron 43-targeting gRNA expression plasmidDepositorInsertgRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6 Polymerase III snRNAAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
AP76_pU.CAG.Cas9-D10A-K848A.rBGpA
Plasmid#199253PurposeExpression construct encoding the high specificity SpCas9-KA-D10A nickaseDepositorInsertHigh specificity S. pyogenes Cas9-D10A-KA nickase
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10A K848APromoterCAG promoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AV13_pCAG.Cas9.gRNA.NT
Plasmid#199260PurposeExpression construct encoding SpCas9 nuclease and a control non-targeting guide RNADepositorInsertsSpCas9 nuclease
Non-targeting (NT) control gRNA
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterCAG promoter and RNA polymerase III promoter for …Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AY23_pgRNA.R5.2
Plasmid#100295PurposeCCR5-targeting gRNA expression plasmidDepositorInsertgRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6 Polymerase III snRNAAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVRa29_371
Plasmid#49684DepositorInsertECF29_371
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS
Plasmid#199252PurposeExpression construct encoding the high specificity eSpCas9(1.1)-D10A nickaseDepositorInsertHigh specificity eSpCas9(1.1)-D10A nickase
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10A K848A K1003A R1060APromoterCAG promoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sema5a-Fc-His
Plasmid#72161PurposeExpresses the extracellular region of the Sema5A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema5a (Sema5a Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema4c-AP-His
Plasmid#72030PurposeExpresses the extracellular region of the Sema4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema4c (Sema4c Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only