We narrowed to 230 results for: Mycobacterium

1. pYC1640

   Plasmid

   #158719

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYC2085

   Plasmid

   #158721

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Plasmid L5 attB::Pleft* mCherry

   Plasmid

   #169411

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   pLeft
   mCherry
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Plasmid L5 attB:Psmyc double blue

   Plasmid

   #170050

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blue

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   mTagBFP2
   CO BFP
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNHEJ-recAmu-sacB

   Plasmid

   #158717

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   recA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R61C

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Plasmid L5 attB:Psmyc double green

   Plasmid

   #169412

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double green

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   mWasabi
   mWasabi CO
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Plasmid L5 attB:Psmyc triple red

   Plasmid

   #169413

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple red

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   mScarlet proper
   COmScarlet
   mRuby3
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJV52

   Plasmid

   #26903

   Depositor

   Graham Hatfull

   Article

   van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

   Insert

   Che9c gene 61
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJV63

   Plasmid

   #26906

   Depositor

   Graham Hatfull

   Article

   van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

   Insert

   Che9c genes 60-61
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pNHEJ-Cas12a-recX

    Plasmid

    #158714

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    recX
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pmyc

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNHEJ-Cas9-recX

    Plasmid

    #158715

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. His-FGE

    Plasmid

    #75147

    Purpose

    Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.

    Depositor

    Richard Fishel

    Article

    Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10

    Insert

    FGE
    

    Use

    Tags

    Histidine tag and Thrombin

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    T7

    Available since

    July 8, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pNHEJ-Cas12a

    Plasmid

    #158713

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1_GEM22-Halo-FRB

    Plasmid

    #197059

    Purpose

    Expression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJV53

    Plasmid

    #26904

    Depositor

    Graham Hatfull

    Article

    van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

    Insert

    Che9c genes 60-61
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMN234

    Plasmid

    #32108

    Depositor

    Michael Niederweis

    Article

    Stephan et al Gene. 2004 Dec 8;343(1):181-90.

    Insert

    flpe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    imyc

    Available since

    Nov. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pINT3

    Plasmid

    #24778

    Depositor

    Tanya Parish

    Article

    Parish et al Tuberculosis (Edinb). 2005 May . 85(3):1

    Type

    Empty backbone

    Use

    Integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMN252

    Plasmid

    #32177

    Depositor

    Michael Niederweis

    Article

    Stephan et al Gene. 2004 Dec 8;343(1):181-90.

    Insert

    rpsL, FRT-hyg-FRT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRVV601

    Plasmid

    #87632

    Purpose

    RMCE Bxb1 attB-flanked vector.

    Depositor

    Richard Mann

    Article

    Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

    Insert

    Bxb1 attB
    

    Use

    Rmce

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p2NIL

    Plasmid

    #20188

    Depositor

    Tanya Parish

    Article

    Parish et al Microbiology (Reading). 2000 Aug;146 ( P

    Type

    Empty backbone

    Use

    Suicide vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge