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pDestTol2-ubi-ASAP1

Plasmid

#121987

Purpose

ubiqutously express ASAP1 voltage biosensor

Depositor

GuangJun Zhang

Article

Silic et al J Vis Exp. 2018 Apr 25;(134). doi: 10.37

Insert

ASAP1

Use

Tol2 transposon destination vector

Tags

Expression

Mutation

Promoter

zebrafish ubiquitin promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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hRAD18 dSAP-EGFP

Plasmid

#68825

Purpose

Expresses human RAD18 (deleting SAP domain) tagged with EGFP

Depositor

Satoshi Tateishi

Article

Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

Insert

human RAD18 (deleting SAP domain) tagged with EGFP (RAD18 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pFasBac+GFP-CAMSAP3

Plasmid

#59038

Purpose

for baculovirus expression of mouse CAMSAP3 in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP3 (Camsap3 Mouse)

Use

Tags

His and eGFP

Expression

Insect

Mutation

Promoter

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRCH-SsAPOBEC3B

Plasmid

#198550

Purpose

Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH-SsAPOBEC3B

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP2s

Plasmid

#101276

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn (human synapsin)

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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TetCMV-Pfv-Sapphire

Plasmid

#203650

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the doxycycline-inducible promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Lentiviral

Tags

Sapphire

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX Sap-SH2

Plasmid

#46477

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Sap (SH2D1A Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 4-128

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-sapphireAKAR-NLS

Plasmid

#118467

Purpose

cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireAKAR-NLS

Use

Tags

Nuclear localization signal (NLS)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-ASAP2s

Plasmid

#101275

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAMKIIa

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-sapphireAKAR

Plasmid

#118466

Purpose

cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

Lyn-sapphireAKAR

Use

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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myc-hRAD18 dSAP

Plasmid

#68830

Purpose

Expresses c-myc-tagged human RAD18 deleting SAP domain

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 deleting SAP domain (RAD18 Human)

Use

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
Anti-SAP102 [N19/2R]

Plasmid

#177510

Purpose

Mammalian Expression Plasmid of anti-SAP102 (Rat). Derived from hybridoma N19/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-SAP102 (Rattus norvegicus) recombinant mouse monoclonal antibody (Dlg3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pFasBac+GFP-CAMSAP2 CC

Plasmid

#59040

Purpose

for baculovirus expression of CAMSAP2 CC in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 CC domain (CAMSAP2 Human)

Use

Tags

His and eGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pFasBac+GFP-CAMSAP3 CC

Plasmid

#59042

Purpose

for baculovirus expression of mouse CAMSAP3 CC in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP3 CC domain (Camsap3 Mouse)

Use

Tags

His and eGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-mCAMSAP3-Myc

Plasmid

#236536

Purpose

For expression of CAMSAP3 (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Flinois et al MicroPubl Biol. 2024 Oct 14;2024:10.1791

Insert

CAMSAP3 (Camsap3 Mouse)

Use

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-pfv-Sapphire

Plasmid

#231957

Purpose

The plasmid containing TRE-pfv-Sapphire for the doxycycline-inducible expression of genetically encoded multimeric nanoparticles (GEM)

Depositor

Michael Shtutman

Article

Korunova et al bioRxiv [Preprint]. 2024 Nov 18:2024.11.

Insert

genetically encoded multimeric nanoparticles

Use

Lentiviral; Doxocycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-Adapter1-SapI

Plasmid

#229200

Purpose

lvl 1 adapter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

T2-T4 - Sap1 adapapter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-Adapter2-SapI

Plasmid

#229201

Purpose

lvl 1 adapter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

T3-T4 - Sap1 adapapter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-SAP102 [N19/2R-2b]

Plasmid

#219474

Purpose

Mammalian Expression Plasmid of anti-SAP102 MAGUK scaffold protein (Rat) subclass-switched IgG2b R-mAb. Derived from hybridoma N19/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-SAP102 MAGUK scaffold protein (Rattus norvegicus) recombinant mouse monoclonal antibody. (Dlg3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-ASAP2

Plasmid

#221303

Purpose

Expression of ASAP2 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

ASAP2 (pLxIS) (Asap2 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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